User:Samiye Yaman/Notebook/Lab 581/2013/02/20: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objections== | |||
==Description== | ==Description== | ||
'''Part 1''' | |||
*Prepare 3 sets of hydrogels with phosphate buffers | |||
A)0.51 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C. | A)0.51 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C. | ||
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C)0.14 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C. | C)0.14 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C. | ||
* | *Homoginize each of the hydrogels solutions with 35 ml of safflower oil. Right after pouring in to a beaker, flash freeze it with liquid nitrogen then place them into freezer. (3 freeze thaw cycle, 20 hours freeze 4 hours room temperature) | ||
'''Part 2''' | |||
*Prepare 3 sets of hydrogels with phosphate buffers | |||
A)0.52 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C. | |||
B)0.25 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C. | |||
C)0.13 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C. | |||
*Homoginize each of the hydrogels solutions with 50 ml of | *Homoginize each of the hydrogels solutions with 50 ml of safflower oil. Pour them in to a petri dish to have a larger surface area then place them into freezer.(-20C) (3 freeze thaw cycle) | ||
'''Part 3''' | |||
*Prepare a set of hydrogel with phosphate buffer | |||
A)0.25 g POVH (MW:89,000-98,000) with 4 ml of phosphate buffer was blaced to a heat block at 100C. | |||
*Homoginize each of the hydrogels solutions with 50 ml of mineral oil. Pour them in to a petri dish to have a larger surface area then place it into the freezer.(-20C) (3 freeze thaw cycle) | |||
==Data== | ==Data== | ||
==Notes== | ==Notes== | ||
*Safflower oil was used instead of mineral oil. Safflower oil has lower freezing point (approximately -17) than mineral oil (fp:-30C) | |||
The reason why we wanted to try safflower oil was it has freezing point lower than -20C. Mineral oil did not freeze completely at -20C. | |||
*We used big petri dishes instead of beakers to increase the surface area and have a less dense medium as hydrogels sink at the bottom of the container. (to observe if larger surface area will make any difference) | |||
*Liquid nitrogen was used to flash freeze the hydrogels before they sink at the bottom of the container. |
Latest revision as of 22:28, 26 September 2017
Experimental Chemistry | Main project page Previous entry Next entry |
ObjectionsDescriptionPart 1
A)0.51 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C. B)0.25 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C. C)0.14 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.
A)0.52 g of PVOH (MW:89,000-98,000) with 4 ml of phosphate buffer was placed on to a heat block at 100C. B)0.25 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C. C)0.13 g of PVOH (MW:89,000-98,000) with 3 ml of phosphate buffer was placed on to a heat block at 100C.
A)0.25 g POVH (MW:89,000-98,000) with 4 ml of phosphate buffer was blaced to a heat block at 100C.
DataNotes
The reason why we wanted to try safflower oil was it has freezing point lower than -20C. Mineral oil did not freeze completely at -20C.
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