User:Samiye Yaman/Notebook/Lab 581/2013/02/11: Difference between revisions
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*0.51 g of PVOH (MW:89000-98000) was placed in to a beaker with 6 ml of phosphate buffer. It was placed in to a hot plate with temperature of 120C.(stir well to dissolve PVOH completely). | *0.51 g of PVOH (MW:89000-98000) was placed in to a beaker with 6 ml of phosphate buffer. It was placed in to a hot plate with temperature of 120C.(stir well to dissolve PVOH completely). | ||
*50ml of mineral oil was placed in to a homogenizer. Add 0.5 g of PVOH with phosphate buffer(prepared at part A) in to the homogenizer and homogenize it for | *50ml of mineral oil was placed in to a homogenizer. Add 0.5 g of PVOH with phosphate buffer(prepared at part A) in to the homogenizer and homogenize it for 1 minutes (slower speed was used to homogenize the mixture). Pour it in to a beaker and place it in to a freezer at -20C for 20 hours.then thaw for 4 hours. 3 freeze-thaw cycle is required. | ||
==Data== | ==Data== | ||
==Notes== | ==Notes== | ||
Revision as of 06:52, 13 February 2013
 Experimental Chemistry
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