User:Samiye Yaman/Notebook/Lab 581/2013/02/06 - Revision history

Samiye Yaman: /* Description */

2013-02-08T20:20:07Z

Description

←Older revision		Revision as of 20:20, 8 February 2013
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	'''Part C'''		'''Part C'''

-	*100ml of mineral oil was placed in to a homogenizer. Add 0.5 g of PVOH with phosphate buffer(prepared at part A) in to the homogenizer and homogenize it for 5 minutes. Pour it in to a beaker and place it in to a freezer at -20C for 20 hours.then thaw for 4 hours.	+	*100ml of mineral oil was placed in to a homogenizer. Add 0.5 g of PVOH with phosphate buffer(prepared at part A) in to the homogenizer and homogenize it for 5 minutes. Pour it in to a beaker and place it in to a freezer at -20C for 20 hours.then thaw for 4 hours. 3 freeze-thaw cycle is required.

	==Data==		==Data==

	==Notes==		==Notes==

Samiye Yaman: /* Description */

2013-02-06T15:49:30Z

Description

←Older revision		Revision as of 15:49, 6 February 2013
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	'''Part C'''		'''Part C'''

-	*100ml of mineral oil was placed in to a homogenizer. Add 0.5 g of PVOH with phosphate buffer(prepared at part A) in to the homogenizer and homogenize it for 5 minutes. Pour it in to a beaker and place it in to a freezer at -20C for 20 hours.	+	*100ml of mineral oil was placed in to a homogenizer. Add 0.5 g of PVOH with phosphate buffer(prepared at part A) in to the homogenizer and homogenize it for 5 minutes. Pour it in to a beaker and place it in to a freezer at -20C for 20 hours.then thaw for 4 hours.

	==Data==		==Data==

	==Notes==		==Notes==

Samiye Yaman: /* Description */

2013-02-06T15:41:58Z

Description

←Older revision		Revision as of 15:41, 6 February 2013
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	*0.51 g of PVOH (MW:89000-98000) was placed in to a beaker with 5 ml of water. It was placed in to a hot plate with temperature of 120C.(stir well to dissolve PVOH completely).Add 2µ of R6G.		*0.51 g of PVOH (MW:89000-98000) was placed in to a beaker with 5 ml of water. It was placed in to a hot plate with temperature of 120C.(stir well to dissolve PVOH completely).Add 2µ of R6G.
		+
		+	'''Part C'''
		+
		+	*100ml of mineral oil was placed in to a homogenizer. Add 0.5 g of PVOH with phosphate buffer(prepared at part A) in to the homogenizer and homogenize it for 5 minutes. Pour it in to a beaker and place it in to a freezer at -20C for 20 hours.

	==Data==		==Data==

	==Notes==		==Notes==

Samiye Yaman: /* Description */

2013-02-06T15:25:43Z

Description

←Older revision		Revision as of 15:25, 6 February 2013
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	'''Part B Dissolving PVOH with phosphate buffer'''		'''Part B Dissolving PVOH with phosphate buffer'''

-	*0.51 g of PVOH (MW:89000-98000) was placed in to a beaker with 3 ml of water. It was placed in to a hot plate with temperature of 120C.(stir well to dissolve PVOH completely)	+	*0.51 g of PVOH (MW:89000-98000) was placed in to a beaker with 5 ml of water. It was placed in to a hot plate with temperature of 120C.(stir well to dissolve PVOH completely).Add 2µ of R6G.

	==Data==		==Data==

	==Notes==		==Notes==

Samiye Yaman: /* Description */

2013-02-06T15:01:47Z

Description

←Older revision		Revision as of 15:01, 6 February 2013
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	*Add 1.25 g of sodium diphosphate and 1.34g of sodium monophosphate in to a 900ml of miliQ water. (Stir well to make sure it is completely homogeneous). Ph needs to be 7.4 so 0.5M of sodium hydroxide was added drop by drop to make sure Ph reaches to 7.4.		*Add 1.25 g of sodium diphosphate and 1.34g of sodium monophosphate in to a 900ml of miliQ water. (Stir well to make sure it is completely homogeneous). Ph needs to be 7.4 so 0.5M of sodium hydroxide was added drop by drop to make sure Ph reaches to 7.4.
		+
		+	'''Part B Dissolving PVOH with phosphate buffer'''
		+
		+	*0.51 g of PVOH (MW:89000-98000) was placed in to a beaker with 3 ml of water. It was placed in to a hot plate with temperature of 120C.(stir well to dissolve PVOH completely)

	==Data==		==Data==

	==Notes==		==Notes==

Samiye Yaman: /* Description */

2013-02-06T14:59:20Z

Description

←Older revision		Revision as of 14:59, 6 February 2013
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	Mass used for acid: (141.96 g/mol) * 0.00885mol= 1.256g		Mass used for acid: (141.96 g/mol) * 0.00885mol= 1.256g
		+
		+	*Add 1.25 g of sodium diphosphate and 1.34g of sodium monophosphate in to a 900ml of miliQ water. (Stir well to make sure it is completely homogeneous). Ph needs to be 7.4 so 0.5M of sodium hydroxide was added drop by drop to make sure Ph reaches to 7.4.

	==Data==		==Data==

	==Notes==		==Notes==

Samiye Yaman: /* Description */

2013-02-06T14:55:42Z

Description

←Older revision		Revision as of 14:55, 6 February 2013
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	* (20mmol-acid)/acid= pH/pka		* (20mmol-acid)/acid= pH/pka

-	(Base/acid)=1.25.	+	(Base/acid)=1.25.

	*Acid=8.85mmol & Base= 11.15mmol		*Acid=8.85mmol & Base= 11.15mmol

Samiye Yaman: /* Description */

2013-02-06T14:55:23Z

Description

←Older revision		Revision as of 14:55, 6 February 2013
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	==Description==		==Description==
		+
		+	*Hydrogels synthesized on 01/02/2013 was transferred from -20C to room temperature.
		+
		+	'''Part A Synthesizing Phosphate Buffer'''
		+
		+	* Concentration Needed; 20mMol
		+	*pH Needed: 7.4
		+	*pka: 7.3m
		+	*base: sodium monophosphate
		+	*acid: sodium diphosphate
		+
		+	*Acid + Base= 20 mmol therefore Base= 20mmol-acid
		+
		+	*pH= pka+ log (base/acid)
		+
		+	* (20mmol-acid)/acid= pH/pka
		+
		+	(Base/acid)=1.25.
		+
		+	*Acid=8.85mmol & Base= 11.15mmol
		+
		+	Mass used for base: (119.98 g/mol)* 0.01115mol= 1.338g
		+
		+	Mass used for acid: (141.96 g/mol) * 0.00885mol= 1.256g

	==Data==		==Data==

	==Notes==		==Notes==

Samiye Yaman: /* Description */

2013-02-06T13:24:17Z

Description

←Older revision		Revision as of 13:24, 6 February 2013
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		+
		+
		+	==Objective==


	==Description==		==Description==
-
-	*The course is designed by American University to experiment different aspects of PVOH films.

-	*During the course PVOH films with different molecular weight will be produced. Effects of cross-linking, addition of different molecules such as gluteraldehyde, different acids, porphrin will be also tested.	+	==Data==

-	*UV-Vis spectrum, Atomic Absorption spectrum, X-Ray, FTIR, DMA, ISE and many other instruments will be used to analyze the results.	+	==Notes==

Samiye Yaman: Autocreate 2013/02/06 Entry for User:Samiye_Yaman/Notebook/Lab_581

2013-02-06T13:21:44Z

Autocreate 2013/02/06 Entry for User:Samiye_Yaman/Notebook/Lab_581

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{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
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==Description==

*The course is designed by American University to experiment different aspects of PVOH films.

*During the course PVOH films with different molecular weight will be produced. Effects of cross-linking, addition of different molecules such as gluteraldehyde, different acids, porphrin will be also tested.

*UV-Vis spectrum, Atomic Absorption spectrum, X-Ray, FTIR, DMA, ISE and many other instruments will be used to analyze the results.