User:Samiye Yaman/Notebook/Lab 581/2013/02/06

Objective

Description

• Hydrogels synthesized on 01/02/2013 was transferred from -20C to room temperature.

Part A Synthesizing Phosphate Buffer

• Concentration Needed; 20mMol
• pH Needed: 7.4
• pka: 7.3m
• base: sodium monophosphate
• acid: sodium diphosphate

• Acid + Base= 20 mmol therefore Base= 20mmol-acid

• pH= pka+ log (base/acid)

• (20mmol-acid)/acid= pH/pka

(Base/acid)=1.25.

• Acid=8.85mmol & Base= 11.15mmol

Mass used for base: (119.98 g/mol)* 0.01115mol= 1.338g

Mass used for acid: (141.96 g/mol) * 0.00885mol= 1.256g

Data

Notes