User:Samiye Yaman/Notebook/Lab 581/2013/02/06: Difference between revisions
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* (20mmol-acid)/acid= pH/pka | * (20mmol-acid)/acid= pH/pka | ||
(Base/acid)=1.25. | |||
*Acid=8.85mmol & Base= 11.15mmol | *Acid=8.85mmol & Base= 11.15mmol | ||
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Mass used for acid: (141.96 g/mol) * 0.00885mol= 1.256g | Mass used for acid: (141.96 g/mol) * 0.00885mol= 1.256g | ||
*Add 1.25 g of sodium diphosphate and 1.34g of sodium monophosphate in to a 900ml of miliQ water. (Stir well to make sure it is completely homogeneous). Ph needs to be 7.4 so 0.5M of sodium hydroxide was added drop by drop to make sure Ph reaches to 7.4. | |||
'''Part B Dissolving PVOH with phosphate buffer''' | |||
*0.51 g of PVOH (MW:89000-98000) was placed in to a beaker with 5 ml of water. It was placed in to a hot plate with temperature of 120C.(stir well to dissolve PVOH completely).Add 2µ of R6G. | |||
'''Part C''' | |||
*100ml of mineral oil was placed in to a homogenizer. Add 0.5 g of PVOH with phosphate buffer(prepared at part A) in to the homogenizer and homogenize it for 5 minutes. Pour it in to a beaker and place it in to a freezer at -20C for 20 hours.then thaw for 4 hours. 3 freeze-thaw cycle is required. | |||
==Data== | ==Data== | ||
==Notes== | ==Notes== |
Revision as of 13:20, 8 February 2013
Experimental Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveDescription
Part A Synthesizing Phosphate Buffer
(Base/acid)=1.25.
Mass used for base: (119.98 g/mol)* 0.01115mol= 1.338g Mass used for acid: (141.96 g/mol) * 0.00885mol= 1.256g
Part B Dissolving PVOH with phosphate buffer
Part C
DataNotes |