User:Samiye Yaman/Notebook/Lab 581/2013/02/06: Difference between revisions

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* (20mmol-acid)/acid= pH/pka
* (20mmol-acid)/acid= pH/pka


        (Base/acid)=1.25.
(Base/acid)=1.25.


*Acid=8.85mmol & Base= 11.15mmol
*Acid=8.85mmol & Base= 11.15mmol

Revision as of 07:55, 6 February 2013

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Objective

Description

  • Hydrogels synthesized on 01/02/2013 was transferred from -20C to room temperature.

Part A Synthesizing Phosphate Buffer

  • Concentration Needed; 20mMol
  • pH Needed: 7.4
  • pka: 7.3m
  • base: sodium monophosphate
  • acid: sodium diphosphate
  • Acid + Base= 20 mmol therefore Base= 20mmol-acid
  • pH= pka+ log (base/acid)
  • (20mmol-acid)/acid= pH/pka

(Base/acid)=1.25.

  • Acid=8.85mmol & Base= 11.15mmol

Mass used for base: (119.98 g/mol)* 0.01115mol= 1.338g

Mass used for acid: (141.96 g/mol) * 0.00885mol= 1.256g

Data

Notes
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