User:Samiye Yaman/Notebook/Lab 581/2013/02/06
From OpenWetWare
(Difference between revisions)
(→Description) |
(→Description) |
||
| Line 15: | Line 15: | ||
==Description== | ==Description== | ||
| + | |||
| + | *Hydrogels synthesized on 01/02/2013 was transferred from -20C to room temperature. | ||
| + | |||
| + | '''Part A Synthesizing Phosphate Buffer''' | ||
| + | |||
| + | * Concentration Needed; 20mMol | ||
| + | *pH Needed: 7.4 | ||
| + | *pka: 7.3m | ||
| + | *base: sodium monophosphate | ||
| + | *acid: sodium diphosphate | ||
| + | |||
| + | *Acid + Base= 20 mmol therefore Base= 20mmol-acid | ||
| + | |||
| + | *pH= pka+ log (base/acid) | ||
| + | |||
| + | * (20mmol-acid)/acid= pH/pka | ||
| + | |||
| + | (Base/acid)=1.25. | ||
| + | |||
| + | *Acid=8.85mmol & Base= 11.15mmol | ||
| + | |||
| + | Mass used for base: (119.98 g/mol)* 0.01115mol= 1.338g | ||
| + | |||
| + | Mass used for acid: (141.96 g/mol) * 0.00885mol= 1.256g | ||
==Data== | ==Data== | ||
==Notes== | ==Notes== | ||
Revision as of 10:55, 6 February 2013
 Experimental Chemistry
|  Main project page Previous entry Next entry
| |
|
ObjectiveDescription
Part A Synthesizing Phosphate Buffer
(Base/acid)=1.25.
Mass used for base: (119.98 g/mol)* 0.01115mol= 1.338g Mass used for acid: (141.96 g/mol) * 0.00885mol= 1.256g DataNotes |
||
