User:Samiye Yaman/Notebook/Lab 581/2013/02/01: Difference between revisions

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'''Part 2: Prepare Hydrogels with 0.5mM of R6G dye''' (for detailed method,  see http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_581/2013/01/30 )
'''Part 2: Prepare Hydrogels with 0.5mM of R6G dye''' (for detailed method,  see http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_581/2013/01/30 )
0.00479 g of R6G dissolved in 10 ml of ethanol.(1mM)
 
take 10 micL and 9.99ml ethanol to make 1micM R6G.
*0.00479 g of R6G dissolved in 10 ml of ethanol(Concentration is 1mM). Take 10 micL and 9.99ml ethanol to make 1micM R6G.


1)0.5g of 22,000 + 6 ml water (A)
1)0.5g of 22,000 + 6 ml water (A)

Revision as of 09:00, 6 February 2013

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Objective

Description

Part 1:Place the hydrogels synthesized on 30/01/2013 in to -20C

Part 2: Prepare Hydrogels with 0.5mM of R6G dye (for detailed method, see http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_581/2013/01/30 )

  • 0.00479 g of R6G dissolved in 10 ml of ethanol(Concentration is 1mM). Take 10 micL and 9.99ml ethanol to make 1micM R6G.

1)0.5g of 22,000 + 6 ml water (A)

2)0.5 of 89,000-98,000 + 6 ml water (C)

3)0.5g of 130,000 + 9 ml of water (E)

4)0.5g of 146,000-186,000 + 9 ml of water (G)

  • add 6 micL of R6G in to no 1) and 2).
  • add 9 micL of R6G in to no 3) and 4).
  • transfer them into cell plates with corresponding letters (A-D) then place the 96 cell plate in to -20C at 4pm.

Data

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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