User:Samiye Yaman/Notebook/Lab 581/2013/01/30: Difference between revisions

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==Description==
==Description==


'''Part 1: Filtration with different kinds of film'''
'''Part 1: Prepare hydrogels'''  


*Film 1: 0.5g PVOH, gluteraldehyde (1ml)
*prepare 2 sets of each


*Film 2: NaMT PVOH
1)0.5g of 22,000 + 6 ml water


*Use 20 ml of CuSO4 during each filtration.
2)0.5 of 89,000-98,000 + 6 ml water


*Filtration done with 3 different timings. 10 minutes, 30 minutes and 1 hour.
3)0.5g of 130,000 + 6 ml of water


4)0.5g of 146,000-186,000 + 6ml of water


'''Part 2'''
*placed into -20C at 10:10 for 1) and 2), 3), and 4) at 10:20.


*DSE measurement for
'''Part 2: Prepare Hydrogels with 0.5mM of R6G dye'''


1) 0.5g PVOH, gluteraldehyde (1ml)
*0.00479 g of R6G dissolved in 10 ml of ethanol.(1mM)


2) 0.5 g PVOH (100000)+HCl+ Succinate acid
*take 10 micL and 9.99ml ethanol to make 1micM R6G.


3) 0.5 g PVOH (22000)+ 3 ml water + Mallic acid + phosphoric acid
1)0.5g of 22,000 + 6 ml water


4) 1 g PVOH (22000) + phosphoric acid + Mallic acid
2)0.5 of 89,000-98,000 + 6 ml water
 
3)0.5g of 130,000 + 6 ml of water
 
4)0.5g of 146,000-186,000 + 6ml of water
 
*add 6micL of R6G in to each hydrogels.
 
*placed into -20C at noon.


==Data==
==Data==

Revision as of 09:45, 30 January 2013

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Objective

Description

Part 1: Prepare hydrogels

  • prepare 2 sets of each

1)0.5g of 22,000 + 6 ml water

2)0.5 of 89,000-98,000 + 6 ml water

3)0.5g of 130,000 + 6 ml of water

4)0.5g of 146,000-186,000 + 6ml of water

  • placed into -20C at 10:10 for 1) and 2), 3), and 4) at 10:20.

Part 2: Prepare Hydrogels with 0.5mM of R6G dye

  • 0.00479 g of R6G dissolved in 10 ml of ethanol.(1mM)
  • take 10 micL and 9.99ml ethanol to make 1micM R6G.

1)0.5g of 22,000 + 6 ml water

2)0.5 of 89,000-98,000 + 6 ml water

3)0.5g of 130,000 + 6 ml of water

4)0.5g of 146,000-186,000 + 6ml of water

  • add 6micL of R6G in to each hydrogels.
  • placed into -20C at noon.

Data

  • Film 1 dissolved during the filtration.

DSE Results

Notes

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