User:Samiye Yaman/Notebook/Lab 581/2013/01/30

Objective

Description

Part 1: Prepare hydrogels

• prepare 2 sets of each

1)0.5g of 22,000 + 6 ml water

2)0.5 of 89,000-98,000 + 6 ml water

3)0.5g of 130,000 + 6 ml of water

4)0.5g of 146,000-186,000 + 6ml of water

• placed into -20C at 10:10 for 1) and 2), 3), and 4) at 10:20.

Part 2: Prepare Hydrogels with 0.5mM of R6G dye

• 0.00479 g of R6G dissolved in 10 ml of ethanol.(1mM)

• take 10 micL and 9.99ml ethanol to make 1micM R6G.

1)0.5g of 22,000 + 6 ml water

2)0.5 of 89,000-98,000 + 6 ml water

3)0.5g of 130,000 + 6 ml of water

4)0.5g of 146,000-186,000 + 6ml of water

• add 6micL of R6G in to each hydrogels.

• placed into -20C at noon.

Data

• Film 1 dissolved during the filtration.

DSE Results

Notes

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