User:Samiye Yaman/Notebook/Lab 581/2012/10/31 - Revision history

Samiye Yaman: /* Data */

Samiye Yaman — Fri, 02 Nov 2012 18:28:44 GMT

Data

←Older revision		Revision as of 18:28, 2 November 2012
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-	'''~~After~~ placing porphyrin film into 1 % HCl solution'''	+	'''Right after placing porphyrin film into 1 % HCl solution'''

	*Edges of the film become more green.		*Edges of the film become more green.

Samiye Yaman: /* Data */

Samiye Yaman — Fri, 02 Nov 2012 18:28:20 GMT

Data

←Older revision		Revision as of 18:28, 2 November 2012
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	[[Image:2012-10-31_11.21.49.jpg\|500px]]		[[Image:2012-10-31_11.21.49.jpg\|500px]]

-	'''48 hours ~~After~~ placing porphyrin film, prepared into 1 % HCl solution'''	+	'''48 hours after placing porphyrin film, prepared into 1 % HCl solution'''

	*The film became full green.(compared to the pictures on 31/10/2012)		*The film became full green.(compared to the pictures on 31/10/2012)

Samiye Yaman: /* Data */

Samiye Yaman — Fri, 02 Nov 2012 16:37:37 GMT

Data

←Older revision		Revision as of 16:37, 2 November 2012
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	[[Image:2012-10-31_11.21.49.jpg\|500px]]		[[Image:2012-10-31_11.21.49.jpg\|500px]]
		+
		+	'''48 hours After placing porphyrin film, prepared into 1 % HCl solution'''
		+
		+	*The film became full green.(compared to the pictures on 31/10/2012)
		+
		+	[[Image:2012-11-02_12.14.36.jpg\|500px]]

	==Notes==		==Notes==

Samiye Yaman: /* Data */

Samiye Yaman — Fri, 02 Nov 2012 16:31:20 GMT

Data

←Older revision		Revision as of 16:31, 2 November 2012
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	[[Image:2012-10-31_11.21.49.jpg\|500px]]		[[Image:2012-10-31_11.21.49.jpg\|500px]]
-
-
-	~~'''Porphyrin film with 2 drops of HCL, prepared on 24/10/2012'''~~
-
-	*Before cross-linking
-
-	*The film shrunk and look more brittle.
-
-	*More intense green color.
-
-	~~[[Image:2012-10-31_11.23.11.jpg\|500px]]~~

	==Notes==		==Notes==

Samiye Yaman: /* Data */

Samiye Yaman — Wed, 31 Oct 2012 16:05:05 GMT

Data

←Older revision		Revision as of 16:05, 31 October 2012
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	'''Porphyrin film with 2 drops of HCL, prepared on 24/10/2012'''		'''Porphyrin film with 2 drops of HCL, prepared on 24/10/2012'''
		+
		+	*Before cross-linking
		+
		+	*The film shrunk and look more brittle.
		+
		+	*More intense green color.

	[[Image:2012-10-31_11.23.11.jpg\|500px]]		[[Image:2012-10-31_11.23.11.jpg\|500px]]

Samiye Yaman: /* Data */

Samiye Yaman — Wed, 31 Oct 2012 16:03:35 GMT

Data

←Older revision		Revision as of 16:03, 31 October 2012
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	[[Image:2012-10-31_11.21.49.jpg\|500px]]		[[Image:2012-10-31_11.21.49.jpg\|500px]]
		+
		+
		+	'''Porphyrin film with 2 drops of HCL, prepared on 24/10/2012'''
		+
		+	[[Image:2012-10-31_11.23.11.jpg\|500px]]

	==Notes==		==Notes==

Samiye Yaman: /* Data */

Samiye Yaman — Wed, 31 Oct 2012 16:01:06 GMT

Data

←Older revision		Revision as of 16:01, 31 October 2012
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	==Data==		==Data==
		+
		+	'''Before placing porphyrin film in to 1 % HCl solution'''
		+	[[Image:2012-10-31_11.19.50.jpg\|500px]]
		+
		+
		+	'''After placing porphyrin film into 1 % HCl solution'''
		+
		+	*Edges of the film become more green.
		+
		+	[[Image:2012-10-31_11.21.49.jpg\|500px]]

	==Notes==		==Notes==

Samiye Yaman: /* Data */

Samiye Yaman — Wed, 31 Oct 2012 15:45:41 GMT

Data

←Older revision		Revision as of 15:45, 31 October 2012
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	==Data==		==Data==
-
-	~~'''ISE results for sodium sulfate dilutions'''~~
-
-	*Dilution 1(1.25ppm)= -180mV
-
-	*Dilution 2(2.5ppm)= -182.6mV
-
-	*Dilution 3(5ppm) = -182.4mV
-
-	*Dilution 4(10ppm )= -177.4mV
-
-	*Dilution 5(20ppm)= -174.6mV
-
-
-	*We decided detection limit was between dilution 1 and 2.
-
-	*More negative number means less sodium ion in the solution.
-
-	*The porphyrin films prepared on 10/10/2012 had a yellowish green color. They were not clear as the other films.

	==Notes==		==Notes==

Samiye Yaman: /* Description */

Samiye Yaman — Wed, 31 Oct 2012 15:45:25 GMT

Description

←Older revision		Revision as of 15:45, 31 October 2012
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	==Description==		==Description==

-	'''Part 1 ~~: Preparation of porphyrin films~~'''	+	'''Part 1 '''

-	* ~~Add 2 drops of~~ HCL~~, 1 ml~~ of ~~gluteraldehyde and 1 ml of porphyrin after the solution is homogeneous and cooled down~~.	+	*Porphyrin film which was cross-linked on 19/10/2012 was placed in to a 1% HCL solution with a volume of 100mL.

-	*Keep everything under the hood.	+	'''Part 2: Prepare a porphyrin solution of 50micM'''

-	'''Part 2 : UV-~~VIS of porphyrin dilutions prepared on 17/10/2012~~'''	+	'''Part 3: UV-Vis'''

-	*~~'''A)''' from the UV-Vis results we concluded that it is necessary to make extra 2 dilutions as dilution 1 and 2 are out~~ of ~~range. They are too concentrated. We would like to obtain a peak lower than~~ 1.	+	*Combination of 1 ml of salt/acid (50micM), 1 ml of 50 micM of porphyrin and 2 ml of HPLC water.
-		+
-	*Dilution 6: 4 ml of ~~dilution 5 + 4 ml of HPLC water.~~(~~0.3375micM)~~	+
-		+
-	*Dilution 7: 4ml of dilution 6 + 4 ml of HPLC water. (0.16785micM)	+
-		+
-	~~'''Part 3 : Prepare dilutions of~~ 50micM ~~Na2SO4, 50micM Al2(SO4~~)3, ~~50micM Fe2(SO4)3''' (HPLC WATER IS USED)~~	+
-		+
-	~~'''Na2SO4'''~~	+
-		+
-	*FW: 142.07 g/ml	+
-		+
-	*1~~/142.07 g/mol x 50micM/L x 10^(-6)mol/1micM x 1/1000ml x 10ml= 0.071035 g in 10~~ ml of ~~HPLC water.~~	+
-		+
-	*(50mM) (x) = (50micM) (10mL)	+
-		+
-	* x = 0.01 ml ~~=10micL~~ of ~~Na2SO4 in 9.99ml~~ HPLC water	+
-		+
-		+
-	~~'''Al2(SO4)3'''~~	+
-		+
-	*FW: 456.~~33g/mol~~	+
-		+
-	*456.33g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.392g in 10 mL H2O.	+
-		+
-	*50micM=10micL of 50mM in 999micL H2O	+
-		+
-		+
-	~~'''Fe2(SO4)3''' (72wt%)~~	+
-		+
-	*FW:399.88g/mol	+
-		+
-	* 0.72 x 399.88g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.144g in 10 mL H2O.	+
-		+
-	*50micM=10micL of 50mM in 999micL H2O	+

	==Data==		==Data==

Samiye Yaman: /* Description */

Samiye Yaman — Wed, 31 Oct 2012 15:38:15 GMT

Description

←Older revision		Revision as of 15:38, 31 October 2012
Line 10:		Line 10:


		+
		+
		+	==Objective==

	==Description==		==Description==
-
-	*The course is designed by American University to experiment different aspects of PVOH films.

-	*~~During~~ the ~~course PVOH films with different molecular weight will be produced~~. ~~Effects~~ of ~~cross~~-~~linking~~, ~~addition~~ of ~~different molecules such~~ as ~~gluteraldehyde, different acids~~, ~~porphrin will~~ be ~~also tested~~.	+	'''Part 1 : Preparation of porphyrin films'''
		+
		+	* Add 2 drops of HCL, 1 ml of gluteraldehyde and 1 ml of porphyrin after the solution is homogeneous and cooled down.
		+
		+	*Keep everything under the hood.
		+
		+	'''Part 2 : UV-VIS of porphyrin dilutions prepared on 17/10/2012'''
		+
		+	*'''A)''' from the UV-Vis results we concluded that it is necessary to make extra 2 dilutions as dilution 1 and 2 are out of range. They are too concentrated. We would like to obtain a peak lower than 1.
		+
		+	*Dilution 6: 4 ml of dilution 5 + 4 ml of HPLC water.(0.3375micM)
		+
		+	*Dilution 7: 4ml of dilution 6 + 4 ml of HPLC water. (0.16785micM)
		+
		+	'''Part 3 : Prepare dilutions of 50micM Na2SO4, 50micM Al2(SO4)3, 50micM Fe2(SO4)3''' (HPLC WATER IS USED)
		+
		+	'''Na2SO4'''
		+
		+	*FW: 142.07 g/ml
		+
		+	*1/142.07 g/mol x 50micM/L x 10^(-6)mol/1micM x 1/1000ml x 10ml= 0.071035 g in 10 ml of HPLC water.
		+
		+	*(50mM) (x) = (50micM) (10mL)
		+
		+	* x = 0.01 ml =10micL of Na2SO4 in 9.99ml HPLC water
		+
		+
		+	'''Al2(SO4)3'''
		+
		+	*FW: 456.33g/mol
		+
		+	*456.33g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.392g in 10 mL H2O.
		+
		+	*50micM=10micL of 50mM in 999micL H2O
		+
		+
		+	'''Fe2(SO4)3''' (72wt%)
		+
		+	*FW:399.88g/mol
		+
		+	* 0.72 x 399.88g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.144g in 10 mL H2O.
		+
		+	*50micM=10micL of 50mM in 999micL H2O
		+
		+	==Data==
		+
		+	'''ISE results for sodium sulfate dilutions'''
		+
		+	*Dilution 1(1.25ppm)= -180mV
		+
		+	*Dilution 2(2.5ppm)= -182.6mV
		+
		+	*Dilution 3(5ppm) = -182.4mV
		+
		+	*Dilution 4(10ppm )= -177.4mV
		+
		+	*Dilution 5(20ppm)= -174.6mV
		+
		+
		+	*We decided detection limit was between dilution 1 and 2.
		+
		+	*More negative number means less sodium ion in the solution.
		+
		+	*The porphyrin films prepared on 10/10/2012 had a yellowish green color. They were not clear as the other films.
		+
		+	==Notes==
		+	This area is for any observations or conclusions that you would like to note.
		+
		+
		+	Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
		+
		+	[[Category:Course]]
		+	[[Category:Miscellaneous]]
		+
		+
		+
		+	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
		+	\|}

-	*UV-Vis spectrum, Atomic Absorption spectrum, X-Ray, FTIR, DMA, ISE and many other instruments will be used to analyze the results.	+	__NOTOC__