User:Samiye Yaman/Notebook/Lab 581/2012/10/31

From OpenWetWare

< User:Samiye Yaman | Notebook | Lab 581 | 2012 | 10(Difference between revisions)
Jump to: navigation, search
(→Description)
Current revision (14:28, 2 November 2012) (view source)
(→Data)
 
(8 intermediate revisions not shown.)
Line 16: Line 16:
==Description==
==Description==
-
'''Part 1 : Preparation of porphyrin films'''
+
'''Part 1 '''
-
* Add 2 drops of HCL, 1 ml of gluteraldehyde and 1 ml of porphyrin after the solution is homogeneous and cooled down.
+
*Porphyrin film which was cross-linked on 19/10/2012 was placed in to a 1% HCL solution with a volume of 100mL.  
-
*Keep everything under the hood.
+
'''Part 2: Prepare a porphyrin solution of 50micM'''
-
'''Part 2 : UV-VIS of porphyrin dilutions prepared on 17/10/2012'''
+
'''Part 3: UV-Vis'''
-
*'''A)''' from the UV-Vis results we concluded that it is necessary to make extra 2 dilutions as dilution 1 and 2 are out of range. They are too concentrated. We would like to obtain a peak lower than 1.
+
*Combination of 1 ml of salt/acid (50micM), 1 ml of 50 micM of porphyrin and 2 ml of HPLC water.
-
 
+
-
*Dilution 6: 4 ml of dilution 5 + 4 ml of HPLC water.(0.3375micM)
+
-
 
+
-
*Dilution 7: 4ml of dilution 6 + 4 ml of HPLC water. (0.16785micM)
+
-
 
+
-
'''Part 3 : Prepare dilutions of 50micM Na2SO4, 50micM Al2(SO4)3, 50micM Fe2(SO4)3''' (HPLC WATER IS USED)
+
-
 
+
-
'''Na2SO4'''
+
-
 
+
-
*FW: 142.07 g/ml
+
-
 
+
-
*1/142.07 g/mol x 50micM/L x 10^(-6)mol/1micM x 1/1000ml x 10ml= 0.071035 g in 10 ml of HPLC water.
+
-
 
+
-
*(50mM) (x) = (50micM) (10mL)
+
-
         
+
-
* x  = 0.01 ml =10micL of Na2SO4 in 9.99ml HPLC water
+
-
 
+
-
 
+
-
'''Al2(SO4)3'''
+
-
 
+
-
*FW: 456.33g/mol
+
-
 
+
-
*456.33g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.392g in 10 mL H2O.
+
-
 
+
-
*50micM=10micL of 50mM in 999micL H2O
+
-
 
+
-
 
+
-
'''Fe2(SO4)3''' (72wt%)
+
-
 
+
-
*FW:399.88g/mol
+
-
 
+
-
* 0.72 x 399.88g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.144g in 10 mL H2O.
+
-
 
+
-
*50micM=10micL of 50mM in 999micL H2O
+
==Data==
==Data==
-
'''ISE results for sodium sulfate dilutions'''
+
'''Before placing porphyrin film in to 1 % HCl solution'''
-
 
+
[[Image:2012-10-31_11.19.50.jpg|500px]]
-
*Dilution 1(1.25ppm)= -180mV
+
-
 
+
-
*Dilution 2(2.5ppm)=  -182.6mV
+
-
*Dilution 3(5ppm)  = -182.4mV
 
-
*Dilution 4(10ppm )= -177.4mV
+
'''Right after placing porphyrin film into 1 % HCl solution'''
-
*Dilution 5(20ppm)= -174.6mV
+
*Edges of the film become more green.
 +
[[Image:2012-10-31_11.21.49.jpg|500px]]
-
*We decided detection limit was between dilution 1 and 2.
+
'''48 hours after placing porphyrin film, prepared into 1 % HCl solution'''
-
*More negative number means less sodium ion in the solution.
+
*The film became full green.(compared to the pictures on 31/10/2012)
-
*The porphyrin films prepared on 10/10/2012 had a yellowish green color. They were not clear as the other films.
+
[[Image:2012-11-02_12.14.36.jpg|500px]]
==Notes==
==Notes==

Current revision

Experimental Chemistry Main project page
Previous entry      Next entry




Objective

Description

Part 1

  • Porphyrin film which was cross-linked on 19/10/2012 was placed in to a 1% HCL solution with a volume of 100mL.

Part 2: Prepare a porphyrin solution of 50micM

Part 3: UV-Vis

  • Combination of 1 ml of salt/acid (50micM), 1 ml of 50 micM of porphyrin and 2 ml of HPLC water.

Data

Before placing porphyrin film in to 1 % HCl solution


Right after placing porphyrin film into 1 % HCl solution

  • Edges of the film become more green.

48 hours after placing porphyrin film, prepared into 1 % HCl solution

  • The film became full green.(compared to the pictures on 31/10/2012)

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



Retrieved from "http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_581/2012/10/31"
Personal tools