User:Samiye Yaman/Notebook/Lab 581/2012/10/31

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(Description)
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==Description==
==Description==
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'''Part 1 : Preparation of porphyrin films'''
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'''Part 1 '''
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* Add 2 drops of HCL, 1 ml of gluteraldehyde and 1 ml of porphyrin after the solution is homogeneous and cooled down.
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*Porphyrin film which was cross-linked on 19/10/2012 was placed in to a 1% HCL solution with a volume of 100mL.  
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*Keep everything under the hood.
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'''Part 2: Prepare a porphyrin solution of 50micM'''
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'''Part 2 : UV-VIS of porphyrin dilutions prepared on 17/10/2012'''
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'''Part 3: UV-Vis'''
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*'''A)''' from the UV-Vis results we concluded that it is necessary to make extra 2 dilutions as dilution 1 and 2 are out of range. They are too concentrated. We would like to obtain a peak lower than 1.
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*Combination of 1 ml of salt/acid (50micM), 1 ml of 50 micM of porphyrin and 2 ml of HPLC water.
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*Dilution 6: 4 ml of dilution 5 + 4 ml of HPLC water.(0.3375micM)
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*Dilution 7: 4ml of dilution 6 + 4 ml of HPLC water. (0.16785micM)
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'''Part 3 : Prepare dilutions of 50micM Na2SO4, 50micM Al2(SO4)3, 50micM Fe2(SO4)3''' (HPLC WATER IS USED)
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'''Na2SO4'''
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*FW: 142.07 g/ml
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*1/142.07 g/mol x 50micM/L x 10^(-6)mol/1micM x 1/1000ml x 10ml= 0.071035 g in 10 ml of HPLC water.
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*(50mM) (x) = (50micM) (10mL)
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* x  = 0.01 ml =10micL of Na2SO4 in 9.99ml HPLC water
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'''Al2(SO4)3'''
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*FW: 456.33g/mol
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*456.33g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.392g in 10 mL H2O.
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*50micM=10micL of 50mM in 999micL H2O
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'''Fe2(SO4)3''' (72wt%)
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*FW:399.88g/mol
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* 0.72 x 399.88g/mol x 50mmol/L x 10^(-3)/1mmol x 1L/1000mL x 10 mml= 0.144g in 10 mL H2O.
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*50micM=10micL of 50mM in 999micL H2O
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==Data==
==Data==

Revision as of 11:45, 31 October 2012

Experimental Chemistry Main project page
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Objective

Description

Part 1

  • Porphyrin film which was cross-linked on 19/10/2012 was placed in to a 1% HCL solution with a volume of 100mL.

Part 2: Prepare a porphyrin solution of 50micM

Part 3: UV-Vis

  • Combination of 1 ml of salt/acid (50micM), 1 ml of 50 micM of porphyrin and 2 ml of HPLC water.

Data

ISE results for sodium sulfate dilutions

  • Dilution 1(1.25ppm)= -180mV
  • Dilution 2(2.5ppm)= -182.6mV
  • Dilution 3(5ppm) = -182.4mV
  • Dilution 4(10ppm )= -177.4mV
  • Dilution 5(20ppm)= -174.6mV


  • We decided detection limit was between dilution 1 and 2.
  • More negative number means less sodium ion in the solution.
  • The porphyrin films prepared on 10/10/2012 had a yellowish green color. They were not clear as the other films.

Notes

This area is for any observations or conclusions that you would like to note.


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