User:Samiye Yaman/Notebook/Lab 571/2012/03/06: Difference between revisions
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==='''Description'''=== | ==='''Description'''=== | ||
'''Effect of Au/BSA Ratio | |||
''' | |||
-Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl4: | |||
--90 Au/BSA | |||
--110 Au/BSA | |||
--130 Au/BSA | |||
--150 Au/BSA | |||
-Dilute to 1 mL with water. | |||
-Place in oven at 80°C. | |||
-Leave solutions in oven for 3.5 hr. | |||
-Centrifuge solutions for 10 min at 13200 rpm. | |||
-Take UV-vis data of supernatant. | |||
'''Effect of Tris Buffer on Protein Fibers''' | |||
-Centrifuge AuNP fibers + 50 mM tris solution made on 02/15/12. | |||
-Take UV-vis absorption data. | |||
-Compare data to that obtained on 02/15/12, 02/21/12, and 02/28/12. | |||
==='''Data'''=== | ==='''Data'''=== |
Revision as of 06:16, 17 April 2012
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ObjectiveDetermining the effect of the mole ratio of Au/BSA on AuNP synthesis DescriptionEffect of Au/BSA Ratio -Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl4: --90 Au/BSA --110 Au/BSA --130 Au/BSA --150 Au/BSA -Dilute to 1 mL with water. -Place in oven at 80°C. -Leave solutions in oven for 3.5 hr. -Centrifuge solutions for 10 min at 13200 rpm. -Take UV-vis data of supernatant. Effect of Tris Buffer on Protein Fibers -Centrifuge AuNP fibers + 50 mM tris solution made on 02/15/12. -Take UV-vis absorption data. -Compare data to that obtained on 02/15/12, 02/21/12, and 02/28/12. Data |