User:Samiye Yaman/Notebook/Lab 571/2012/02/01: Difference between revisions

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==Description==
==Description==
'''Part A'''
Syntsizing 2 solutions of AuNP with different ratio:


---Solution 1 (Au/BSA mole ratio = 70)
*UV-vis data of following solutions were taken:


*Combine 1 mL of 3.66 mM HAuCl4 and 3.02 mL of 0.0173 mM BSA in a test tube.


*Dilute to 10 mL with water.
1) ratio of  70 (Au to BSA)(prepared on 31/01/12)


*Place in oven.(80°C)


---Solution 2 (Au/BSA mole ratio = 166)
2) ratio of 166 (Au to BSA)(prepared on 31/01/12)


*Combine 1 mL of 3.66 mM HAuCl4 and 1.27 mL of 0.0173 mM BSA in a test tube.


*Dilute to 10 mL with water.
3) dilutions of 0.9 ml of each AuNP solution with 0.1 ml of tris dilutions with a pH 10.01.


*Place in oven. (80°C)
-50mM


'''Part B'''
-5.0mM
Leave solutions in oven for 2.5 hr.
 
-0.50mM
 
-0.05mM
 
 
*UV-vis data of 0.9 mL 70 Au/BSA ratio + 0.1 mL 50 mM tris buffer solution will be taken every wednesday.


==Data==
==Data==

Revision as of 12:17, 29 February 2012

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Determining Processes of Isolating Gold Nanoparticles Using Refolding Buffers

Objective

Determining the effects of tris buffer with a pH 10.1 on gold nanoparticle and protein fibers.

Description

  • UV-vis data of following solutions were taken:


1) ratio of 70 (Au to BSA)(prepared on 31/01/12)


2) ratio of 166 (Au to BSA)(prepared on 31/01/12)


3) dilutions of 0.9 ml of each AuNP solution with 0.1 ml of tris dilutions with a pH 10.01.

-50mM

-5.0mM

-0.50mM

-0.05mM


  • UV-vis data of 0.9 mL 70 Au/BSA ratio + 0.1 mL 50 mM tris buffer solution will be taken every wednesday.

Data

Ratio:70, purple solution 


Ratio:166, purple fibers

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.

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