Construct Luminescence Measurement
Induction of Apoptosis
Apoptosis was induced by 10μM staurosporine in half of the samples. Samples will be measured after 4hr and 6hr incubations at 37C. A Promega Luciferase Assay will be used.
4hr
Construct |
+ |
-
|
1X Gaussia |
4,143,449 |
19,643,584
|
5X Gaussia |
1,283,243 |
8,496,529
|
1X Renilla |
203,756,023 |
231
|
5X Renilla |
220,700,532 |
1,302,498,176
|
No DNA |
232 |
242
|
|
<bars title="Log10 Luminescence"
colors=336699,669933,993366
ylabel=10 xlabel legend grid=xy
ymin=0 ymax=10 size=450x300>
,Apoptotic,Non-Apoptotic
1xG, 6.617361998, 7.293220728
5xG, 6.108308904, 6.929241544
1xR, 8.309110455, 2.36361198
5xR, 8.34380338, 9.114777124
No DNA, 2.365487985, 2.383815366
</bars>
|
6hr
Construct |
+ |
-
|
1X Gaussia |
397 |
26,226,718
|
5X Gaussia |
1,755,862 |
9,029,151
|
1X Renilla |
115,874,928 |
1,035,950,080
|
5X Renilla |
112,831,944 |
568,635,136
|
No DNA |
367 |
448
|
|
<bars title="Log10 Luminescence"
colors=336699,669933,993366
ylabel=10 xlabel legend grid=xy
ymin=0 ymax=10 size=450x300>
,Apoptotic,Non-Apoptotic
1xG, 2.598790507, 7.418743947
5xG, 6.24449038, 6.955646916
1xR, 8.063989477, 9.015338828
5xR, 8.052432071, 8.754833691
No DNA, 2.564666064, 2.651278014
</bars>
|
Conclusions
- Seems backwards
- Perhaps enzymes are being degraded
- Significant cell death was seen through microscope in both induced and uninduced samples
|