User:Robwarden/Notebook/Caspase-3 Activatable Probe/2009/10/05: Difference between revisions
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===6hr=== | ===6hr=== | ||
===4hr=== | |||
{| | |||
| | |||
{| {{table}} | |||
! Construct || + || - | |||
|- | |||
|1X Gaussia || 4,143,449 || 19,643,584 | |||
|- | |||
|5X Gaussia || 1,283,243 || 8,496,529 | |||
|- | |||
|1X Renilla || 203,756,023 || 231 | |||
|- | |||
|5X Renilla || 220,700,532 || 1,302,498,176 | |||
|- | |||
|No DNA || 232 || 242 | |||
|} | |||
|<bars title="Log10 Luminescence" | |||
colors=336699,669933,993366 | |||
ylabel=10 xlabel legend grid=xy | |||
ymin=0 ymax=10 size=450x300> | |||
,Apoptotic,Non-Apoptotic | |||
1xG, 6.617361998, 7.293220728 | |||
5xG, 6.108308904, 6.929241544 | |||
1xR, 8.309110455, 2.36361198 | |||
5xR, 8.34380338, 9.114777124 | |||
No DNA, 2.365487985, 2.383815366 | |||
</bars> | |||
|} | |||
===Conclusions=== | ===Conclusions=== | ||
*Seems backwards | *Seems backwards |
Revision as of 13:29, 5 October 2009
Caspase-3 Activatable Probe | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html> | ||||||||||||||||||||||||||||||||||||||||
Construct Luminescence MeasurementInduction of ApoptosisApoptosis was induced by 10μM staurosporine in half of the samples. Samples will be measured after 4hr and 6hr incubations at 37C. A Promega Luciferase Assay will be used. 4hr
6hr4hr
Conclusions
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