User:Robwarden/Notebook/Caspase-3 Activatable Probe/2009/09/14: Difference between revisions
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== | ==Colony Screen== | ||
===Miniprep=== | |||
with Qiagen Kit | |||
===Digestion=== | |||
*EcoRI & SgrAI (.5 μL each) | |||
*10 μL colony DNA (or 1 μg control plasmid) | |||
*NEB Buffer 4 | |||
===Gel Electrophoresis=== | |||
{| {{table}} | |||
|+ '''Top Wells''' | |||
!Lane | |||
|1 || 2 || 3 || 4 || 5 || 6 || 7 || 8 || 9 || 10 || 11 || 12 || 13 || 14 || 15 | |||
|- | |||
!Sample | |||
|5x Control || 1kb || R-5-1 || R-5-2 || R-5-3 || R-5-4 || R-5-5 || R-5-6 || 100 bp || G-5-1 || G-5-2 || G-5-3 || G-5-4 || G-5-5 || G-5-6 | |||
|} | |||
{| {{table}} | |||
|+ '''Bottom Wells''' | |||
!Lane | |||
| 1 || 2 || 3 || 4 || 5 || 6 || 7 || 8 | |||
|- | |||
!Sample | |||
|1x Control || 1kb || R-1-1 || R-1-2 || R-1-3 || R-1-4 || R-1-5 || R-1-6 | |||
|} | |||
*Naming Scheme: | |||
**G = hgLuc, R = hRLuc8 | |||
**1 = 1xDEVD, 5 = 5xDEVD | |||
**Final Number = Colony Number | |||
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Revision as of 07:14, 15 September 2009
Caspase-3 Activatable Probe | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||
Colony ScreenMiniprepwith Qiagen Kit Digestion
Gel Electrophoresis
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