User:Roberta Diaz Jimenez/Notebook/CHEM 472/2016/04/06: Difference between revisions
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*Note: on March 30, loaded 10ul onto the membrane filter paper discs which resulted in significantly decreased inhibition very likely due to such low volume. Loading 15µL and note if there is still inhibition so as to lessen the load on the filter paper discs from 20µL as well as increase load from 10µL | *Note: on March 30, loaded 10ul onto the membrane filter paper discs which resulted in significantly decreased inhibition very likely due to such low volume. Loading 15µL and note if there is still inhibition so as to lessen the load on the filter paper discs from 20µL as well as increase load from 10µL | ||
*Note: that this technique was conducted on the benchtop. Between each loading of a disc on a plate, the tweezers used were washed with EtOH to minimize contamination. However, the plates were open to the air for a period of time during plating and loading of discs. Because we are testing for bacterial inhibition by the nanoparticles and control, if there is contamination, but inhibition we may never know that there was contamination in the first place. Discussed with Dr. Hartings and protocol was approved. | *Note: that this technique was conducted on the benchtop. Between each loading of a disc on a plate, the tweezers used were washed with EtOH to minimize contamination. However, the plates were open to the air for a period of time during plating and loading of discs. Because we are testing for bacterial inhibition by the nanoparticles and control, if there is contamination, but inhibition we may never know that there was contamination in the first place. Discussed with Dr. Hartings and protocol was approved. | ||
==Results== | ==Results== |
Revision as of 13:33, 4 May 2016
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ObjectivePerform fourth agar diffusion disc assay with increased volume loaded onto discs. Materials
Procedure
Results |