User:Roberta Diaz Jimenez/Notebook/CHEM 472/2016/03/29: Difference between revisions

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==Objectives==
==Objective==
# Make agar to plate petri dishes
Data analysis for reactions run on March 15
# Make another set of serial dilutions
# Plate petri dishes


==Materials==
==Data Workup protocol for all Main UV-VIS Machine analysis and Fluorescence Spectroscopy==
# Agar mix
UVVis
# Water
#import data into excel
# 30 eppendorf tubes
#Blank sample data by subtracting water blank absorbance values for before and after reaction
# Three 100mL falcon tubes
#Absorbance against wavelength
# Protein-nanoparticle solutions
# Monitor maximum absorbance wavelengths before and after
# Ampicilin
#monitor absorbance wavelengths
# 200µL pipette
# 2.8L flask
# Hot plate
# Two 1L screw-cap bottles
 
==Methods==
*Making the agar and plating
#
*Making the dilutions
# Label eppendorf tubes
 
==Results==


Fluorescence
#import data into excel
#subtract water blank sample data from nanoparticle sample data (before and after)
#compute integral for each sample from between 305nm-470nm (peak size and cut off ends to avoid noise in integral calculation)
# calculate ratios of before and after of integrals for same same (just before and after)
# monitor fluorescence peak emission wavelengths




Revision as of 14:01, 4 May 2016

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Objective

Data analysis for reactions run on March 15

Data Workup protocol for all Main UV-VIS Machine analysis and Fluorescence Spectroscopy

UVVis

  1. import data into excel
  2. Blank sample data by subtracting water blank absorbance values for before and after reaction
  3. Absorbance against wavelength
  4. Monitor maximum absorbance wavelengths before and after
  5. monitor absorbance wavelengths

Fluorescence

  1. import data into excel
  2. subtract water blank sample data from nanoparticle sample data (before and after)
  3. compute integral for each sample from between 305nm-470nm (peak size and cut off ends to avoid noise in integral calculation)
  4. calculate ratios of before and after of integrals for same same (just before and after)
  5. monitor fluorescence peak emission wavelengths



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