User:Roberta Diaz Jimenez/Notebook/CHEM 472/2016/02/02: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
Line 12: Line 12:
*Metal and Fructose Stock Solutions
*Metal and Fructose Stock Solutions
#Label three falcon tubes to make the concentrated solutions.  
#Label three falcon tubes to make the concentrated solutions.  
#


*Protein Stock Solutions
*Protein Stock Solutions
#Labelled falcon tubes for Casein, Whey, Soy, Rice, and Pea proteins.
#Labelled falcon tubes for Casein, Whey, Soy, Rice, and Pea proteins.
#Filled each tube with 10mLs of water.
#Added 5.78mg of casein into its tube.
#


*Sample Prep
*Sample Prep
#Label eppendorf tubes 1 through 64.
#Label eppendorf tubes 1 through 64.
#Follow the following tables:
'''Table 1.''' Protocol to make gold and silver samples using Casein as protein
{| {{table}}
| align="center" style="background:#f0f0f0;"|''' Sample # '''
| align="center" style="background:#f0f0f0;"|''' 2.5mM HAuCl4 (µL)'''
| align="center" style="background:#f0f0f0;"|''' 2.5mM AgNO3 (µL)'''
| align="center" style="background:#f0f0f0;"|''' Protein (µL)'''
| align="center" style="background:#f0f0f0;"|''' 2.5mM Fructose (µL)'''
| align="center" style="background:#f0f0f0;"|''' Water (µL)'''
| align="center" style="background:#f0f0f0;"|''' Au/Ag : Protein'''
| align="center" style="background:#f0f0f0;"|''' Notes'''
|-
|1||40||0||560||0||400||1:15||
|-
|2||40||0||440||0||520||1:11||
|-
|3||40||0||360||0||600||1:09||
|-
|4||40||0||280||0||680||1:07||
|-
|5||40||0||200||0||760||1:05||
|-
|6||0||40||560||0||600||1:15||
|-
|7||0||40||360||0||580||1:09||
|-
|8||0||40||200||0||560||1:05||
|}


'''Table 2.''' Protocol to make gold and silver samples using other five proteins
*Detailed procedure can be found [https://docs.google.com/spreadsheets/d/1XIncoYXZyIeGlpAFkbnr44U3PQ61jNbax-hkexNerUM/edit#gid=303734626 SRB Lab Notebook Data] for this entry's date
{| {{table}}
| align="center" style="background:#f0f0f0;"|''' Sample # '''
| align="center" style="background:#f0f0f0;"|''' 2.5mM HAuCl4 (µL)'''
| align="center" style="background:#f0f0f0;"|''' 2.5mM AgNO3 (µL)'''
| align="center" style="background:#f0f0f0;"|''' Protein (µL)'''
| align="center" style="background:#f0f0f0;"|''' 2.5mM Fructose (µL)'''
| align="center" style="background:#f0f0f0;"|''' Water (µL)'''
| align="center" style="background:#f0f0f0;"|''' Au/Ag : Protein'''
| align="center" style="background:#f0f0f0;"|''' Notes'''
|-
|9||40||0||560||0||400||1:15||
|-
|10||40||0||440||0||520||1:11||
|-
|11||40||0||360||0||600||1:09||
|-
|12||40||0||280||0||680||1:07||
|-
|13||40||0||200||0||760||1:05||
|-
|14||0||40||560||0||400||
|-
|15||0||40||560||20||380||
|-
|16||0||40||560||40||360||
|-
|17||0||40||360||0||600||
|-
|18||0||40||360||20||580||
|-
|19||0||40||360||40||560||
|-
|20||0||40||200||0||760||
|-
|21||0||40||200||20||740||
|-
|22||0||40||200||40||720||


==Results==
==Results==

Latest revision as of 01:29, 27 September 2017

Project name Main project page
Previous entry      Next entry

Objective

Set up reactions for our five proteins with silver and gold in order to obtain nanoparticles. Make stock solutions for both the metals, fructose, and the five proteins.

Procedure

  • Metal and Fructose Stock Solutions
  1. Label three falcon tubes to make the concentrated solutions.
  • Protein Stock Solutions
  1. Labelled falcon tubes for Casein, Whey, Soy, Rice, and Pea proteins.
  • Sample Prep
  1. Label eppendorf tubes 1 through 64.

Results


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Roberta_Diaz_Jimenez/Notebook/CHEM_472/2016/02/02&oldid=1017919"