User:Roberta Diaz Jimenez/Notebook/CHEM 471 Experimental Lab/2015/10/07

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Objective

Measure protease Trypsin kinetics with Bradford Assay using 1µM of trypsin.

Procedure

  • Protease Sample Prep.
  1. Used eppendorf tube no. 11 that weighed 1.02097g, and contained 0.00154g.
  2. Added 1mL of phosphate/CaCl2 buffer.
  3. Final concentration: 66.09µM
  • Sample Prep.
  1. Used 7 eppendorf tubes, each containing gold fibers.
  2. To each tube add:
    1. 0.985mL of buffer
    2. 0.015mL of trypsin (add this at the time of putting the tubes in the 37˚C hot water bath).
  • Blank Prep.
  1. In on eppendorf tube add:
    1. 0.985mL of tris buffer
    2. 0.015mL of trypsin solution

Data


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