User:Roberta Diaz Jimenez/Notebook/CHEM 471 Experimental Lab/2015/10/07: Difference between revisions
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== | ==Objective== | ||
* | Measure protease Trypsin kinetics with Bradford Assay using 1µM of trypsin. | ||
==Procedure== | |||
*Protease Sample Prep. | |||
#Used eppendorf tube no. 11 that weighed 1.02097g, and contained 0.00154g. | |||
#Added 1mL of phosphate/CaCl2 buffer. | |||
#Final concentration: 66.09µM | |||
*Sample Prep. | |||
#Used 7 eppendorf tubes, each containing gold fibers. | |||
#To each tube add: | |||
##0.985mL of buffer | |||
##0.015mL of trypsin (add this at the time of putting the tubes in the 37˚C hot water bath). | |||
*Blank Prep. | |||
#In on eppendorf tube add: | |||
##0.985mL of tris buffer | |||
##0.015mL of trypsin solution | |||
==Data== | |||
Revision as of 10:22, 13 October 2015
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ObjectiveMeasure protease Trypsin kinetics with Bradford Assay using 1µM of trypsin. Procedure
Data |