User:Roberta Diaz Jimenez/Notebook/CHEM 471 Experimental Lab/2015/09/29: Difference between revisions
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[[Image:Bradford_Assay_TrypsinandAUfibers_09_29.png|700px]] | [[Image:Bradford_Assay_TrypsinandAUfibers_09_29.png|700px]] | ||
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__NOTOC__ | __NOTOC__ |
Revision as of 11:02, 13 October 2015
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ObjectivePerform a Bradford Analysis to analyze the degradation of protease trypsin. ProcedureIn order to do the Bradford Assay of Protease Degradation, we followed Dr. Hartings' protocol, and did the following specific steps:
Calculations: V1 = [(1µM)(1mL)]/46.35µM = 0.02157mL, amount of trypsin solution needed Volume of buffer: 1mL - 0.02157mL = 0.9784mL Data |