User:Roberta Diaz Jimenez/Notebook/CHEM 471 Experimental Lab/2015/09/15: Difference between revisions
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##The rest of the volume was filled with water: 1 - (1mL fibers + 0.3mL buffer + 0.0573mL trypsin)= 1.643mL water. | ##The rest of the volume was filled with water: 1 - (1mL fibers + 0.3mL buffer + 0.0573mL trypsin)= 1.643mL water. | ||
##The trypsin was added last, at the second reading such that second reading becomes time 0. | ##The trypsin was added last, at the second reading such that second reading becomes time 0. | ||
#We ran our sample for | #We ran our sample for 2hrs30mins. | ||
#Readings were taken every 2 minutes and uploaded directly to DropBox for later analysis. | #Readings were taken every 2 minutes and uploaded directly to DropBox for later analysis. | ||
Calculations for volume of trypsin: | Calculations for volume of trypsin: | ||
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#To each tube, add approximately 1mg of trypsin, weigh and record. | #To each tube, add approximately 1mg of trypsin, weigh and record. | ||
#Calculate the concentration in each tube if 1mL of liquid were added. | #Calculate the concentration in each tube if 1mL of liquid were added. | ||
Calculations to | Calculations to | ||
==Data== | ==Data== |
Revision as of 10:18, 6 October 2015
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ObjectiveFor this lab session, our group (GRP 3) will be doing oceanography and prepping samples with our assigned protease: trypsin. After completing oceanography readings, we will also redo stock solutions to have gold fibers instead of nanoparticles, and prepare more trypsin samples for later use. Procedure
Calculations for volume of trypsin: C1·V1 = C2·V2, where C1 = 52.36µM C2 = 1µM V2 = 3mL V1 = 57.3µL
Calculations to DataTable 1. Measurements taken while making the trypsin sample tubes
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