User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/10/19

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Spermatogenesis

  • Collect virgins for control and experimental crosses
  • Dissect control and experimental
      • Note: Control all had bright yellow testis, but no other parts were different.
    • Fix for 30' in 4% formaldehyde
    • Wash 3x5' in PBS-T
    • Block ON in 2% NGS