User:Rebeca Rodriguez/Notebook/Chem 581/2014/10/29

Biomaterials Design Lab

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Objective Begin bead synthesis Determine which method works best to cross link beads Description Procedure Weigh out approximately 1.0000g of PVA and 0.1100g of clay 1.0004g of PVA and 0.1100g of clay This is mixed with 7mL of 10% of HCl. The HCl should not react with the acetone. Using a burette, 100mL of acetone:7.14mL of of gluteraldehyde are added. Drops of the PVA and clay mixture will be added in order to create beads. The burette will allow a longer time to elapse for the beads to cross link with the gluteraldehyde. Using a pasteur pipette, the PVA and clay mixture will be added drop wise into the burette with acetone and gluteraldehyde. When dropping the mixture into the burette, beads did not form. Some clumped together in an obscure ball and took the shape of a thinker and whispy fiber. It almost looked like a thinker floss. Because acetone was not sufficient to create beads, so it was replaced with hexane. Hexane with a couple drops of glutaraldehyde was added to a petri dish and drops of the PVA and clay mixture were added. This created beads. However if the beads touched each other, they would clump together to create a big bead. Time must elapse in order for one to take the beads out so that they do not clump together. Data Add data and results here... Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.