User:Rebeca Rodriguez/Notebook/Chem 471/2016/03/01

Biomaterials Design Lab

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Objective Take UV Vis data of the 24 hour samples of 25% red nanoparticles and 25% purple nanoparticles, made with DI water, that were set up last Wednesday. Take UV Vis data of stock solutions after a serial dilution of both the lysozyme nanoparticles, made with DI water, and the purple nanoparticles, made with spring water, made last Wednesday Create lysozyme nanoparticles with spring water instead Set up plants in 25% lysozyme nanoparticles, made with DI water, and 25% purple nanoparticles, made with spring water. Protocol UV Vis of Stock Solutions Four serial dilutions (half dilution) were made of the lysozyme nanoparticles, synthesized on 02/22/2016, and were run on UV-Vis. The same dilutions were performed using the purple nanoparticles made with spring water. NP Uptake Setup 25% solutions of both the lysozyme nanoparticles with normal water and purple AuNPs with spring water (synthesized on 02/22/2016). Ferns with similarly extensive root systems were added to the solutions and were analyzed after 2 hours and 24 hours via UV Vis. Lysozyme Nanoparticle Setup 100mL stock solution was made using the following steps: 15.21mg of lysozyme was added to 25mL of water in a volumetric flask (42.55mM). The solutions was mixed and added to the beaker. 0.84mL of 1% by weight HAuCl4 stock solution was added (made on 02/23/2016) 74.16mL of water was added, bringing the final solution volume up to 100mL. The solution was then covered with foil and placed in the oven for 4 hours at 80 degrees celsius. Results UV-Vis data was taken of the 25% solutions red and purple citrate gold nanoparticles, made with regular water, that set up for uptake with java ferns for 24 hours last week. Figure 1: UV-Vis spectra for 25% Red Citrate AuNPs Figure 2: UV-Vis spectra for Lysozyme AuNPs in Spring Water Figure 3: UV-Vis spectra for 25% Purple Citrate AuNPs