User:Rebeca Rodriguez/Notebook/Chem 471/2015/11/10: Difference between revisions
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## Emission from 400 to 650 nm | ## Emission from 400 to 650 nm | ||
Directions also taken from [[User:Michael S. Bible/Notebook/CHEM-671/690 Lab Notebook/2015/11/10|Michael Bible]] | Directions also taken from [[User:Michael S. Bible/Notebook/CHEM-671/690 Lab Notebook/2015/11/10|Michael Bible]]. | ||
==Data== | |||
The spectra were then integrated from 410 nm to 650 nm on Excel by using the midpoint method and then converted to concentration values using the calibration curve created on [[User:Michael_S._Bible/Notebook/CHEM-671/690_Lab_Notebook/2015/09/29|9/29]]. | |||
[[Image:Fluorescense_Spectra_Nov_10.png|1000px]] | |||
[[Image:Fluorescense_Conc_v_Time_Nov_10.png|1000px]] | |||
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Revision as of 16:28, 23 November 2015
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Fluorescence Analysis of Protease DegradationWe are using the Pierce quantitative fluorometric peptide assay. (product link here) The general procedure for today was taken from Dr. Hartings's Notebook All samples will be incubated in eppendorf tubes, which will be placed in the 37 °C water bath on the shaker. Samples should all contain the same concentration of materials. You should make samples for measurements at 10 minutes, 15 minutes, 30 minutes, 45 minutes, 1 hr, 1.5 hr, 2 hr.
Directions also taken from Michael Bible. DataThe spectra were then integrated from 410 nm to 650 nm on Excel by using the midpoint method and then converted to concentration values using the calibration curve created on 9/29. |