User:Rebeca Rodriguez/Notebook/Chem 471/2015/10/13: Difference between revisions
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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Objective== | |||
#Run Ocean Optics Kinetics on AuNP Fibers by 1 uM proteinase K. | |||
#Data analysis! | |||
==Protocol== | |||
5 1 mL AuNP fiber samples were centrifuged (and supernatant fluid removed), and combined into one 1 mL sample in Tris/NaCl buffer solution. A small volume of proteinase K was added such that the the volume of proteinase K in the cuvette was 1 uM. The degradation of the AuNP fibers was then monitored using the Ocean Optics UV-Vis. Spectra were taken every 2 minutes, with one spectrum taken before the addition of the proteinase K. | |||
Set up protocols for the Ocean Optics UV-Vis can be found [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2015/09/08|here]]. | |||
==Data== | ==Data== | ||
[[ | [[Image:Ocean_Optics_10-13.png|1000px]] | ||
This is the spectra for the first 60 minutes of the degradation of AuNP fibers by proteinase K. | |||
[[Image:Ocean_Optics_Analysis_10-13.png|1000px]] | |||
This figure displays the peak absorbance values from the first spectra absorbance plotted time. | |||
Revision as of 16:08, 23 November 2015
 Project name
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Objective
Protocol5 1 mL AuNP fiber samples were centrifuged (and supernatant fluid removed), and combined into one 1 mL sample in Tris/NaCl buffer solution. A small volume of proteinase K was added such that the the volume of proteinase K in the cuvette was 1 uM. The degradation of the AuNP fibers was then monitored using the Ocean Optics UV-Vis. Spectra were taken every 2 minutes, with one spectrum taken before the addition of the proteinase K. Set up protocols for the Ocean Optics UV-Vis can be found here. Data
This is the spectra for the first 60 minutes of the degradation of AuNP fibers by proteinase K.
This figure displays the peak absorbance values from the first spectra absorbance plotted time.
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