User:Rebeca Rodriguez/Notebook/Chem 471/2015/10/07: Difference between revisions
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Latest revision as of 01:20, 27 September 2017
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Bradford Analysis of Protease DegradationThe general procedure today was taken from Dr. Hartings's notebook. All samples will be incubated in eppendorf tubes, which will be placed in the 37°C water bath on the shaker. Samples should all contain the same concentration of materials. You should make samples for measurements at 10 minutes, 15 minutes, 30 minutes, 45 minutes, 1 hr, 1.5 hr, 2 hr. (Note, this is for the first time you do this. As you get better, I will expect you to get more measurements done).
DataThe absorbance versus time of the spectra shows variation. It is still unclear as to what this variation is due to. |