User:Rebeca Rodriguez/Notebook/581/2014/09/17: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Objective==
==Objective==
Learn how to maintain an OpenWetWare Notebook.
* UV-vis of the 2,8,80,and 200 ppm PVA film solutions.  
 
* Finishing the exfoliated clay synthesis
==Description==
* Centrifuge the cation exchange synthesis
# Add experimental record here. Include what, how, and why...
* pXRD of the PVA-films from last class
 
==Data==
====UVis for Malachite Green====
[[Image:UVis_9.17.png]]
 
This graph shows the expected absorbance of malachite green versus the wavelength in nanometers.
 
====UVis for PVA films====
[[Image:UVis2_9.17.png]]
 
This graph shows the amount of malachite green absorbed after a PVA film was added to the solution versus the wavelength in nanometers. The 80 ppm and 200 ppm were diluted 50x and 100x respectively.
 
====Calibration Curve for 425 nm====
[[Image:Calibration_Curve_425.png]]
 
This graph shows the expected absorbency of malachite green versus the concentration at 425 nm.
====Calibration Curve for 615 nm====
[[Image:Calibration_Curve_615.png]]


This graph shows the expected absorbency of malachite green versus the concentration at 615 nm.
==Protocol==
====Finish Synthesis of exfoliated clays====
*This procedure can be found on Friday, August 29th. After completion the films will be stored in water.  


==Notes==
====UV-vis of PVA films soaked in malachite green====
This area is for any observations or conclusions that you would like to note.


*The solutions we were able to measure the absorbance of 2ppm and the 8ppm without diluting, for the 80ppm we did a 50X dilution in which we put 0.5mL in a 10mL volumetric and for the 200ppm we did a 100X dilution in which we did 0.1mL into a 10mL volumetric


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
====Centrifugation of Cation exchange clays====
# Mass out exactly 27.42g into a 50mL flacon tube
# Centrifuge at 4,000RPM for 30minutes at 4 degrees Celsius 
# Collect supernatant (dry on watch glass)
# Wash with water then centrifuge again
# Wash with ethanol and centrifuge again
# Repeat steps 4 and 5 again 


[[Category:Course]]
*Washing with water allows for the removal of any leftover sodium ions
[[Category:Miscellaneous]]
*Washing with ethanol removes surfactants






[[Category:Course]]
[[Category:Miscellaneous]]


*pXRD Preparation which can be seen on Friday, September 12th


==Data==
====UV-vis Absorption of the 2ppm and 8ppm Malachite Green Solutions====
[[Image:UVis_9.17.png|750px]]
<br>
The purpose of this was to figure out how much of the Malachite Green was absorbed into the glass of the vials for each concentration. Our data focuses on the 2ppm and the 8ppm. [[User:Madeleine Y. Bee/Notebook/CHEM-581 Experimental Chemistry/2014/09/12|Madeline's]] group tested the 200ppm and the 80ppm, [[User:James C. Schwabacher/Notebook/CHEM-571/2014/09/12|James's]] group 200ppm and 2ppm, [[User:Michael S. Bible/Notebook/581/2014/09/12|Michael's]] group did 80ppm and 8ppm


====UV-vis Absorption of the 2, 8, 80, and 200ppm Malachite Green Solutions with PVA films====
[[Image:UVis2_9.17.png|750px]]
<br>
This is able to give us an accurate representation of how much Malachite green is being absorbed into the film based off the difference in absorption spectra. After the amount absorbed into the glass is calculated we will be able to get an even more accurate value of how much Malachite Green is being absorbed into the film.


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Latest revision as of 00:18, 27 September 2017

Project name Main project page
Previous entry      Next entry

Objective

  • UV-vis of the 2,8,80,and 200 ppm PVA film solutions.
  • Finishing the exfoliated clay synthesis
  • Centrifuge the cation exchange synthesis
  • pXRD of the PVA-films from last class

Protocol

Finish Synthesis of exfoliated clays

  • This procedure can be found on Friday, August 29th. After completion the films will be stored in water.

UV-vis of PVA films soaked in malachite green

  • The solutions we were able to measure the absorbance of 2ppm and the 8ppm without diluting, for the 80ppm we did a 50X dilution in which we put 0.5mL in a 10mL volumetric and for the 200ppm we did a 100X dilution in which we did 0.1mL into a 10mL volumetric

Centrifugation of Cation exchange clays

  1. Mass out exactly 27.42g into a 50mL flacon tube
  2. Centrifuge at 4,000RPM for 30minutes at 4 degrees Celsius
  3. Collect supernatant (dry on watch glass)
  4. Wash with water then centrifuge again
  5. Wash with ethanol and centrifuge again
  6. Repeat steps 4 and 5 again
  • Washing with water allows for the removal of any leftover sodium ions
  • Washing with ethanol removes surfactants



  • pXRD Preparation which can be seen on Friday, September 12th

Data

UV-vis Absorption of the 2ppm and 8ppm Malachite Green Solutions


The purpose of this was to figure out how much of the Malachite Green was absorbed into the glass of the vials for each concentration. Our data focuses on the 2ppm and the 8ppm. Madeline's group tested the 200ppm and the 80ppm, James's group 200ppm and 2ppm, Michael's group did 80ppm and 8ppm

UV-vis Absorption of the 2, 8, 80, and 200ppm Malachite Green Solutions with PVA films


This is able to give us an accurate representation of how much Malachite green is being absorbed into the film based off the difference in absorption spectra. After the amount absorbed into the glass is calculated we will be able to get an even more accurate value of how much Malachite Green is being absorbed into the film.



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