User:Ramalldf/Notebook/Research/2009/03/13: Difference between revisions
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== | ==3/13/09== | ||
* | |||
*I have the complete set of notes in my notebook, but this is just to be able to share with you. | |||
===AscI Restriction Digest of TOPO Clones=== | |||
*I digested with AscI just like before, and ran a gel using both new and old [http://tools.invitrogen.com/content/sfs/productnotes/f_050719_1kbplus%20dna%20ladder-ts-tl-mkt-hl.pdf Kb + ladders]. | |||
*Here is the content of each lane (10 ul of between 70-200 ng/ul for each digested sample): | |||
[[Image:Diegogel3-14-09.PNG||thumb|600px|This is the digest that Diego did today. It looks like we cloned something in there! Except I don't know why the heavy bands on the double digested samples are larger than the bands on the uncut samples. Looking at it again, I'm thinking that it might've come out clearer if I took the gel off of the tray when I took the picture.]] | |||
**Top (wt): | |||
***L1: New kb+ ladder | |||
***L2:Uncut WT plasmid (sample 6) | |||
***L3:Empty | |||
***L4:Control 1 | |||
***L5:Empty | |||
***L6:Digest sample 1 | |||
***L7:Empty | |||
***L8:Digest sample 3 | |||
***L9:Empty | |||
***L10:Digest sample 6 | |||
***L11:Empty | |||
***L12:Digest sample 9 | |||
***L13:Empty | |||
***L14:Old kb+ ladder | |||
**Bottom(L99 Mut): | |||
***L1: New kb+ ladder | |||
***L2:Uncut Mut plasmid (sample 6) | |||
***L3:Empty | |||
***L4:Digest sample 1 | |||
***L5:Empty | |||
***L6:Digest sample 3 | |||
***L7:Empty | |||
***L8:Digest sample 6 | |||
***L9:Empty | |||
***L10:Digest sample 9 | |||
***L11:Empty | |||
***L12:Control 2 | |||
***L13:Empty | |||
***L14:Old kb+ ladder | |||
*By the controls, I mean the set of rxns where we did the polishing step without any of our DNA in there that we later cloned, transformed, miniprep-ed and now digested. | |||
*Let me know what you think! (email if you don't have an openwetware account). | |||
Revision as of 16:30, 13 March 2009
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3/13/09
AscI Restriction Digest of TOPO Clones
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