User:Radoslaw Kwasniak/Notebook/Transcription factor delivery through T3SS/2012/07/11: Difference between revisions

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* BBA_K11309 => 30μl DNA
* BBA_K11309 => 30μl DNA


==PCR - test==
Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan))


Reaction
*
Conditions


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Revision as of 10:53, 12 July 2012

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Overnight growth check

  • BBA_B0030 (good - turbid)
  • BBA_K11309 (good - turbid)

Miniprep

Used QIA Gen

  • BBA_B0030 => 30μl DNA
  • BBA_K11309 => 30μl DNA

PCR - test

Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan))

Reaction

Conditions


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