User:Rachel Mary Jones/Notebook/Biology 210 at AU: Difference between revisions
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After observing the transect various samples of nature were collected and used to form a hay infusion, this hay infusion was left to process over the course of a week | After observing the transect various samples of nature were collected and used to form a hay infusion, this hay infusion was left to process over the course of a week | ||
1/29/16 | |||
In this week’s lab, we used the Agar plates to identify and study bacteria. | |||
Hay Infusion observation: | |||
The water in the hay infusion is a stagnant murky brown. It has a bit of a musty smell. Sediment has settled around the sides of the infusion as well as at the bottom. I suspect that the smell has worsened due to the growth of the bacteria in the infusion. | |||
Agar plate observations: | |||
As I was in a different lab last week, I am catching up on observing the Agar plates. There appears to be bacterial growth on most of the plates (except 10^-7), even with the antibiotics. Two of the plates have dark black growth, however this is due to them being created on blood agar plates. The rest of the Agar plates have yellow and white clusters of bacteria. When the lid of the agar plates are removed, there is a very foul smell coming from the bacteria. | |||
There are different clusters and colonies of bacteria on the agar plates. The most on the 10^-3 without tet plate. As the dilution gets stronger, we see less and less colonies. We can also see that plates with the antibiotic had significantly fewer colonies, indicting that the antibiotic has an effect. The fact that bacteria still grew all the way up to 10^-5 dilution with tetracycline, indicts this is bacteria that is somewhat resistant to antibiotics. | |||
Determine the mechanisms of action for tetracycline and the types of bacteria that are sensitive to this tetracycline. Cite your sources! | |||
Wet mount observations | |||
Gram Stain observations: | |||
Samples from 10^-3 with tete, 10^-5, 10^-5 with tet and 10^-7 were created and observed under the microscope. All of these slides showed different types of bacteria growth. In | |||
Revision as of 06:39, 5 February 2016
1/15/2016
Transect 3
Description: This transect is located in the garden area at american university. It contains both abiotic and biotic life. The area has 3 sections of garden with 2 walkways and 3 benches throughout
abiotic life: Trees: 8 tall trees, 7 short trees Bushes: 16 long grassy brown bushes shrubs: 14 short interwoven bushes throughout no rocks
Biotic life -1 birds nest in a tree -1 squirel on the ground
See photo for transect diagram
After observing the transect various samples of nature were collected and used to form a hay infusion, this hay infusion was left to process over the course of a week
1/29/16
In this week’s lab, we used the Agar plates to identify and study bacteria.
Hay Infusion observation: The water in the hay infusion is a stagnant murky brown. It has a bit of a musty smell. Sediment has settled around the sides of the infusion as well as at the bottom. I suspect that the smell has worsened due to the growth of the bacteria in the infusion.
Agar plate observations: As I was in a different lab last week, I am catching up on observing the Agar plates. There appears to be bacterial growth on most of the plates (except 10^-7), even with the antibiotics. Two of the plates have dark black growth, however this is due to them being created on blood agar plates. The rest of the Agar plates have yellow and white clusters of bacteria. When the lid of the agar plates are removed, there is a very foul smell coming from the bacteria.
There are different clusters and colonies of bacteria on the agar plates. The most on the 10^-3 without tet plate. As the dilution gets stronger, we see less and less colonies. We can also see that plates with the antibiotic had significantly fewer colonies, indicting that the antibiotic has an effect. The fact that bacteria still grew all the way up to 10^-5 dilution with tetracycline, indicts this is bacteria that is somewhat resistant to antibiotics.
Determine the mechanisms of action for tetracycline and the types of bacteria that are sensitive to this tetracycline. Cite your sources!
Wet mount observations
Gram Stain observations:
Samples from 10^-3 with tete, 10^-5, 10^-5 with tet and 10^-7 were created and observed under the microscope. All of these slides showed different types of bacteria growth. In
