User:Puja Mody/Notebook/Magnetite Shenanigans/2014/10/08: Difference between revisions
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== | ==Magnetite synthesis using stirring== | ||
* | *In order to make hydrogels more uniform in size, an overhead stirrer was used during synthesis instead of the sonicator method used in the general [[Magnetite hydrogel synthesis|Protocol]] | ||
==Procedure== | |||
#Heat a hot water bath to 80°C. | |||
#Follow the [[Magnetite hydrogel synthesis|Magnetite Protocol Synthesis]] and make 1 sample. | |||
*DO NOT ADD IRON TO THE SAMPLE YET. | |||
#attach sample to the overhead stirrer contraption. ensure that the falcon tube containing the sample is submerged in the hot water bath and that the stirrer is in the solution. | |||
#set the stirrer to 4000rpm and begin stirring the solution. | |||
#As the sample is being stirred, add the 2mL of FeSO<sub>4</sub> drop-wise to the falcon tube. | |||
#Allow the sample to react for 4 hours. check on the samples often to make sure that the stirrer is not causing the sample solution to overflow out of the tube. | |||
==Analysis== | |||
*Every 30 minutes collect 2mL samples of the synthesizing solution for microscope analysis. | |||
#remove 1mL of sample and place in eppendorf tube. Remove another 1mL sample and place in a different eppendorf tube. Label the tubes with how many minutes since synthesis began (30,60, 90, 120 etc). | |||
#Centrifuge down the samples for 4-5 minutes. Remove supernatant and leave pellet behind. | |||
#Add 1mL of deionized water to each of the samples and re-suspend the magnetite samples by vortexing | |||
#remove a few drops of sample and look at under a microscope. | |||
#repeat this process every 30 minutes for 4 hours. | |||
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Revision as of 11:36, 14 October 2014
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Magnetite synthesis using stirring
Procedure
Analysis
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