User:Puja Mody/Notebook/Magnetite Shenanigans/2014/09/19: Difference between revisions

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**real concentration of R6G used: 398.7 uM
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Real concentration of Rhodamine used'''
| align="center" style="background:#f0f0f0;"|''''''
|-
| concentration (M)||0.005
|-
| volume stock (L)||0.1
|}
 
*Total sample volume: 1 mL
 
 
Dye addition to samples was done at a range of concentrations from 20 μM - 200 μM
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample Number'''
| align="center" style="background:#f0f0f0;"|'''Conc. Dye in each sample (M)'''
| align="center" style="background:#f0f0f0;"|'''volume of dye added'''
| align="center" style="background:#f0f0f0;"|'''in uL'''
|-
| 1||0.00002||0.000004||4
|-
| 2||0.00004||0.000008||8
|-
| 3||0.00006||0.000012||12
|-
| 4||0.00008||0.000016||16
|-
| 5||0.0001||0.00002||20
|-
| 6||0.00015||0.00003||30
|-
| 7||0.0002||0.00004||40
|}
 
 
 
 
 
 
==NOTES==
** From UV-Vis spectra collections, it was determined that the true concentration of the R6G that was used was: 398.7 uM


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Revision as of 12:51, 23 September 2014

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Dye Addition

  • Preparation of Rhodamine 6G stock solution
Rhodamine 6G '
Molecular Weight (g/mol) 479.02
mass(g) 2.3951
volume stock (L) 0.01
Concentration (M) 0.5
Real concentration of Rhodamine used '
concentration (M) 0.005
volume stock (L) 0.1
  • Total sample volume: 1 mL


Dye addition to samples was done at a range of concentrations from 20 μM - 200 μM

Sample Number Conc. Dye in each sample (M) volume of dye added in uL
1 0.00002 0.000004 4
2 0.00004 0.000008 8
3 0.00006 0.000012 12
4 0.00008 0.000016 16
5 0.0001 0.00002 20
6 0.00015 0.00003 30
7 0.0002 0.00004 40




NOTES

    • From UV-Vis spectra collections, it was determined that the true concentration of the R6G that was used was: 398.7 uM


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