User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/28 - Revision history

Puja Mody at 17:56, 7 December 2012

Puja Mody — Fri, 07 Dec 2012 17:56:06 GMT

←Older revision		Revision as of 17:56, 7 December 2012
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	*The Au/ADA solutions had not shown fibers at mole ratio 25 but did show fibers at mole ratio 50. in order to better pinpoint a mole ratio that gave gold nanoparticles in solution, we remade Au/ADA solutions at mole ratios 10-45 (going up by intervals of 5 making a total of 8 solutions), using the same gold stock and the same ADA (Frac #8).		*The Au/ADA solutions had not shown fibers at mole ratio 25 but did show fibers at mole ratio 50. in order to better pinpoint a mole ratio that gave gold nanoparticles in solution, we remade Au/ADA solutions at mole ratios 10-45 (going up by intervals of 5 making a total of 8 solutions), using the same gold stock and the same ADA (Frac #8).
	* These samples were then capped with aluminum foil and placed in an oven for 4 hours at 80°C.		* These samples were then capped with aluminum foil and placed in an oven for 4 hours at 80°C.
		+
		+
		+	==DATA==
		+	[[Image:Postdialysis.jpg]]

	==Conclusions==		==Conclusions==
-	* For images of the UV-VIs spectras for all runs, please see Mary's notebook.
	* In the Au/ADA spectra, no peaks were seen in the λ500nm range, instead, a slight peak was seen around λ250-325. What this peak represents, we are not sure.		* In the Au/ADA spectra, no peaks were seen in the λ500nm range, instead, a slight peak was seen around λ250-325. What this peak represents, we are not sure.
	* For the newly made Au/ADA solutions at mole ratios 10-45, some of the samples produces a slight purple tinge prior to its placement in the oven. As a result, a UV-Vis spectrum of these samples ( mole ratios 10, 15, 40, 45) was taken. For full details on the makings of these samples and the data collected, please see [[User:Michael F. Nagle/Notebook/Chem 571/2012/11/29\| Michaels Notebook]]. His results showed that in the 25-33 mole ratio range, purple solutions were present without fibers. Further zeroing in on this range needs to be done in the fugure in order to more specifically determine the mole ratio of Au to ADA that leads to the formation of nanoparticles in solution.		* For the newly made Au/ADA solutions at mole ratios 10-45, some of the samples produces a slight purple tinge prior to its placement in the oven. As a result, a UV-Vis spectrum of these samples ( mole ratios 10, 15, 40, 45) was taken. For full details on the makings of these samples and the data collected, please see [[User:Michael F. Nagle/Notebook/Chem 571/2012/11/29\| Michaels Notebook]]. His results showed that in the 25-33 mole ratio range, purple solutions were present without fibers. Further zeroing in on this range needs to be done in the fugure in order to more specifically determine the mole ratio of Au to ADA that leads to the formation of nanoparticles in solution.

Puja Mody: /* Procedure */

Puja Mody — Fri, 07 Dec 2012 17:55:11 GMT

Procedure

←Older revision		Revision as of 17:55, 7 December 2012
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	*The Au/ADA solutions had not shown fibers at mole ratio 25 but did show fibers at mole ratio 50. in order to better pinpoint a mole ratio that gave gold nanoparticles in solution, we remade Au/ADA solutions at mole ratios 10-45 (going up by intervals of 5 making a total of 8 solutions), using the same gold stock and the same ADA (Frac #8).		*The Au/ADA solutions had not shown fibers at mole ratio 25 but did show fibers at mole ratio 50. in order to better pinpoint a mole ratio that gave gold nanoparticles in solution, we remade Au/ADA solutions at mole ratios 10-45 (going up by intervals of 5 making a total of 8 solutions), using the same gold stock and the same ADA (Frac #8).
-	* These samples were then capped with aluminum foil and placed in an oven for 4 hours at 80°C.	+	* These samples were then capped with aluminum foil and placed in an oven for 4 hours at 80°C.

	==Conclusions==		==Conclusions==

Puja Mody: /* Conclusions */

Puja Mody — Fri, 07 Dec 2012 17:54:26 GMT

Conclusions

←Older revision		Revision as of 17:54, 7 December 2012
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	* For images of the UV-VIs spectras for all runs, please see Mary's notebook.		* For images of the UV-VIs spectras for all runs, please see Mary's notebook.
	* In the Au/ADA spectra, no peaks were seen in the λ500nm range, instead, a slight peak was seen around λ250-325. What this peak represents, we are not sure.		* In the Au/ADA spectra, no peaks were seen in the λ500nm range, instead, a slight peak was seen around λ250-325. What this peak represents, we are not sure.
-	* For the newly made Au/ADA solutions at mole ratios 10-45, some of the samples produces a slight purple tinge prior to its placement in the oven. As a result, a UV-Vis spectrum of these samples ( mole ratios 10, 15, 40, 45) was taken. For full details on the makings of these samples, please see Michaels Notebook	+	* For the newly made Au/ADA solutions at mole ratios 10-45, some of the samples produces a slight purple tinge prior to its placement in the oven. As a result, a UV-Vis spectrum of these samples ( mole ratios 10, 15, 40, 45) was taken. For full details on the makings of these samples and the data collected, please see [[User:Michael F. Nagle/Notebook/Chem 571/2012/11/29\| Michaels Notebook]]. His results showed that in the 25-33 mole ratio range, purple solutions were present without fibers. Further zeroing in on this range needs to be done in the fugure in order to more specifically determine the mole ratio of Au to ADA that leads to the formation of nanoparticles in solution.
	*		*

Puja Mody at 18:34, 29 November 2012

Puja Mody — Thu, 29 Nov 2012 18:34:53 GMT

←Older revision		Revision as of 18:34, 29 November 2012
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	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==UV-Vis Everything==
-	* ~~Insert content here~~...	+
		+	==Goals==
		+	* Run UV-Vis on Dialysis Au/ADA solutions
		+	*Dissolve/ resuspend fibers in solutions using tris buffer
		+	* Make new Au/ADA solutions
		+
		+	==Procedure==
		+	*The Au/ADA solutions made [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/27\| yesterday]] produced fibers at most mole ratios except for mole ratio 25. We ran a UV-Vis of 1mL of each of the samples in plastic cuvettes After running the samples through the instrument, each sample was then repipetted back into it's test tube.
		+	*We then took 1mL of .1M tris buffer at pH 9.99 made on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/04\| 2012/09/04]]. We chose this pH because a quick search of the literature showed that the optimum pH for Tris and ADA was between pH 8-9. Therefore, in order to destabilize the ADA and cause it to unfold once again and get nanoparticles in solution, we chose the higher pH with the hopes that it would do what we were hoping for.
		+	* The Au/ADA solutions had 2 mL of solutions to which we added 1mL of tris buffer into each solution. We shook the test tubes a little to mix the Tris in and let the samples sit for 10 minutes.
		+	* After 10 minutes, fibers had not resuspended and there was no visual difference in the solutions, As a result, 1mL more of the tris buffer was added to the samples. and 10 more minutes were allowed to pass.
		+	* Though not much visual difference was still noted, a UV-Vis spectra of the 'resuspended' fibers were taken. Some of the sample solutions looked resuspended and others did not. those that did not were mole ratios 75, 100, 125.
		+
		+	*The Au/ADA solutions had not shown fibers at mole ratio 25 but did show fibers at mole ratio 50. in order to better pinpoint a mole ratio that gave gold nanoparticles in solution, we remade Au/ADA solutions at mole ratios 10-45 (going up by intervals of 5 making a total of 8 solutions), using the same gold stock and the same ADA (Frac #8).
		+	* These samples were then capped with aluminum foil and placed in an oven for 4 hours at 80°C.
		+
		+	==Conclusions==
		+	* For images of the UV-VIs spectras for all runs, please see Mary's notebook.
		+	* In the Au/ADA spectra, no peaks were seen in the λ500nm range, instead, a slight peak was seen around λ250-325. What this peak represents, we are not sure.
		+	* For the newly made Au/ADA solutions at mole ratios 10-45, some of the samples produces a slight purple tinge prior to its placement in the oven. As a result, a UV-Vis spectrum of these samples ( mole ratios 10, 15, 40, 45) was taken. For full details on the makings of these samples, please see Michaels Notebook
		+	*
		+

Puja Mody: Autocreate 2012/11/28 Entry for User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles

Puja Mody — Thu, 29 Nov 2012 18:15:49 GMT

Autocreate 2012/11/28 Entry for User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles

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==Entry title==
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