User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/27 - Revision history

Puja Mody at 17:58, 7 December 2012

2012-12-07T17:58:25Z

←Older revision		Revision as of 17:58, 7 December 2012
Line 17:		Line 17:
	* [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>]] made on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17\| October 17]] was used for the solutions. 8.42mM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>]] solution and 54μM ADA were used to prepare solutions with 1μM ADA and 25μM, 50μM, 75μM, 100μM, 125μM, 150μM, 175μM, and 200μM HAuCl<sub>4</sub>. Water was used as a solvent and enough was added for each solution to be 2mL. The amount of ADA used was kept constant at 55.19μL. The solutions were capped with tin foil and heated for 4 hours at 80<sup>o</sup>C.		* [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>]] made on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17\| October 17]] was used for the solutions. 8.42mM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>]] solution and 54μM ADA were used to prepare solutions with 1μM ADA and 25μM, 50μM, 75μM, 100μM, 125μM, 150μM, 175μM, and 200μM HAuCl<sub>4</sub>. Water was used as a solvent and enough was added for each solution to be 2mL. The amount of ADA used was kept constant at 55.19μL. The solutions were capped with tin foil and heated for 4 hours at 80<sup>o</sup>C.

-	* Meanwhile we continued to run our ADA Kinetic Assay. We continued to monitor λ 235 but instead of correlating it with adenosine, we monitored the kinetics as the creation of inosine. Please see Mary's notebook for ~~data~~. in order to ensure that the absorbance remained below 1, we had to use half as much of the ADA	+	* Meanwhile we continued to run our ADA Kinetic Assay. We continued to monitor λ 235 but instead of correlating it with adenosine, we monitored the kinetics as the creation of inosine. Please see [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/27\| Mary's notebook]] for graphs of the spectra. in order to ensure that the absorbance remained below 1, we had to use half as much of the ADA

	==Conclusions==		==Conclusions==
-	* The Au/ADA solutions made on [[User:Michael F. Nagle/Notebook/Chem 571/2012/11/14\| November 14]] were run through the UV-Vis by Mike on Tuesday. Please see his notebook for details on procedure and more observations. The solutions did not produce any nano particle fibers and the no absorbance was detected in the UV-Vis.	+	* The Au/ADA solutions made on [[User:Michael F. Nagle/Notebook/Chem 571/2012/11/14\| November 14]] were run through the UV-Vis by Mike on [[User:Michael F. Nagle/Notebook/Chem 571/2012/11/20\| Tuesday]]. Please see his notebook for details on procedure and more observations. The solutions did not produce any nano particle fibers and the no absorbance was detected in the UV-Vis.

Puja Mody: /* Procedure */

2012-11-29T18:18:41Z

Procedure

←Older revision		Revision as of 18:18, 29 November 2012
Line 15:		Line 15:
	* The contents of the dialysis pouches were collected in separate falcon tubes. The two samples of ADA that were run through the dialysis were Frac #2 and Frac #8 from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26\| 2012/09/26]]. We decided to made the new Au/ADA solutions using ADA frac # 8. We chose it because after New frac, it was the collected fraction that had the highest [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/07\| concentration of ADA present]].		* The contents of the dialysis pouches were collected in separate falcon tubes. The two samples of ADA that were run through the dialysis were Frac #2 and Frac #8 from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26\| 2012/09/26]]. We decided to made the new Au/ADA solutions using ADA frac # 8. We chose it because after New frac, it was the collected fraction that had the highest [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/07\| concentration of ADA present]].

-	* [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>] made on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17\| October 17]] was used for the solutions. 8.42mM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>]] solution and 54μM ADA were used to prepare solutions with 1μM ADA and 25μM, 50μM, 75μM, 100μM, 125μM, 150μM, 175μM, and 200μM HAuCl<sub>4</sub>. Water was used as a solvent and enough was added for each solution to be 2mL. The amount of ADA used was kept constant at 55.19μL. The solutions were capped with tin foil and heated for 4 hours at 80<sup>o</sup>C.	+	* [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>]] made on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17\| October 17]] was used for the solutions. 8.42mM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>]] solution and 54μM ADA were used to prepare solutions with 1μM ADA and 25μM, 50μM, 75μM, 100μM, 125μM, 150μM, 175μM, and 200μM HAuCl<sub>4</sub>. Water was used as a solvent and enough was added for each solution to be 2mL. The amount of ADA used was kept constant at 55.19μL. The solutions were capped with tin foil and heated for 4 hours at 80<sup>o</sup>C.

	* Meanwhile we continued to run our ADA Kinetic Assay. We continued to monitor λ 235 but instead of correlating it with adenosine, we monitored the kinetics as the creation of inosine. Please see Mary's notebook for data. in order to ensure that the absorbance remained below 1, we had to use half as much of the ADA		* Meanwhile we continued to run our ADA Kinetic Assay. We continued to monitor λ 235 but instead of correlating it with adenosine, we monitored the kinetics as the creation of inosine. Please see Mary's notebook for data. in order to ensure that the absorbance remained below 1, we had to use half as much of the ADA

Puja Mody: /* Procedure */

2012-11-29T18:15:38Z

Procedure

←Older revision		Revision as of 18:15, 29 November 2012
Line 17:		Line 17:
	* [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>] made on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17\| October 17]] was used for the solutions. 8.42mM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>]] solution and 54μM ADA were used to prepare solutions with 1μM ADA and 25μM, 50μM, 75μM, 100μM, 125μM, 150μM, 175μM, and 200μM HAuCl<sub>4</sub>. Water was used as a solvent and enough was added for each solution to be 2mL. The amount of ADA used was kept constant at 55.19μL. The solutions were capped with tin foil and heated for 4 hours at 80<sup>o</sup>C.		* [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>] made on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17\| October 17]] was used for the solutions. 8.42mM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>]] solution and 54μM ADA were used to prepare solutions with 1μM ADA and 25μM, 50μM, 75μM, 100μM, 125μM, 150μM, 175μM, and 200μM HAuCl<sub>4</sub>. Water was used as a solvent and enough was added for each solution to be 2mL. The amount of ADA used was kept constant at 55.19μL. The solutions were capped with tin foil and heated for 4 hours at 80<sup>o</sup>C.

-	* Meanwhile we continued to run our ADA Kinetic Assay. We continued to monitor λ 235 but instead of correlating it with adenosine, we monitored the kinetics as the creation of inosine. Please see Mary's notebook for data ~~and data analysis~~.	+	* Meanwhile we continued to run our ADA Kinetic Assay. We continued to monitor λ 235 but instead of correlating it with adenosine, we monitored the kinetics as the creation of inosine. Please see Mary's notebook for data. in order to ensure that the absorbance remained below 1, we had to use half as much of the ADA

	==Conclusions==		==Conclusions==

Puja Mody: /* Procedure */

2012-11-27T19:42:21Z

Procedure

←Older revision		Revision as of 19:42, 27 November 2012
Line 17:		Line 17:
	* [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>] made on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17\| October 17]] was used for the solutions. 8.42mM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>]] solution and 54μM ADA were used to prepare solutions with 1μM ADA and 25μM, 50μM, 75μM, 100μM, 125μM, 150μM, 175μM, and 200μM HAuCl<sub>4</sub>. Water was used as a solvent and enough was added for each solution to be 2mL. The amount of ADA used was kept constant at 55.19μL. The solutions were capped with tin foil and heated for 4 hours at 80<sup>o</sup>C.		* [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>] made on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17\| October 17]] was used for the solutions. 8.42mM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>]] solution and 54μM ADA were used to prepare solutions with 1μM ADA and 25μM, 50μM, 75μM, 100μM, 125μM, 150μM, 175μM, and 200μM HAuCl<sub>4</sub>. Water was used as a solvent and enough was added for each solution to be 2mL. The amount of ADA used was kept constant at 55.19μL. The solutions were capped with tin foil and heated for 4 hours at 80<sup>o</sup>C.

-	* Meanwhile we continued to run our ADA Kinetic Assay. We continued to monitor λ 235 but instead of correlating it with adenosine, we monitored the kinetics as the creation of inosine.	+	* Meanwhile we continued to run our ADA Kinetic Assay. We continued to monitor λ 235 but instead of correlating it with adenosine, we monitored the kinetics as the creation of inosine. Please see Mary's notebook for data and data analysis.

	==Conclusions==		==Conclusions==

Puja Mody at 19:41, 27 November 2012

2012-11-27T19:41:31Z

←Older revision		Revision as of 19:41, 27 November 2012
Line 18:		Line 18:

	* Meanwhile we continued to run our ADA Kinetic Assay. We continued to monitor λ 235 but instead of correlating it with adenosine, we monitored the kinetics as the creation of inosine.		* Meanwhile we continued to run our ADA Kinetic Assay. We continued to monitor λ 235 but instead of correlating it with adenosine, we monitored the kinetics as the creation of inosine.
		+
		+	==Conclusions==
		+	* The Au/ADA solutions made on [[User:Michael F. Nagle/Notebook/Chem 571/2012/11/14\| November 14]] were run through the UV-Vis by Mike on Tuesday. Please see his notebook for details on procedure and more observations. The solutions did not produce any nano particle fibers and the no absorbance was detected in the UV-Vis.

Puja Mody: /* Entry title */

2012-11-27T19:37:53Z

Entry title

←Older revision		Revision as of 19:37, 27 November 2012
Line 6:		Line 6:
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Kinetics and Dialysis==
-	* ~~Insert content here~~...	+
		+	==Goals==
		+	* Make Au and ADA solutions with the ADA that was placed through dialysis [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/14\| two weeks ago]]
		+	* Run Completed ADA kinetic assay using Newfrac ADA
		+
		+	==Procedure==
		+	* The contents of the dialysis pouches were collected in separate falcon tubes. The two samples of ADA that were run through the dialysis were Frac #2 and Frac #8 from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26\| 2012/09/26]]. We decided to made the new Au/ADA solutions using ADA frac # 8. We chose it because after New frac, it was the collected fraction that had the highest [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/07\| concentration of ADA present]].
		+
		+	* [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>] made on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17\| October 17]] was used for the solutions. 8.42mM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4\|HAuCl<sub>4</sub>]] solution and 54μM ADA were used to prepare solutions with 1μM ADA and 25μM, 50μM, 75μM, 100μM, 125μM, 150μM, 175μM, and 200μM HAuCl<sub>4</sub>. Water was used as a solvent and enough was added for each solution to be 2mL. The amount of ADA used was kept constant at 55.19μL. The solutions were capped with tin foil and heated for 4 hours at 80<sup>o</sup>C.
		+
		+	* Meanwhile we continued to run our ADA Kinetic Assay. We continued to monitor λ 235 but instead of correlating it with adenosine, we monitored the kinetics as the creation of inosine.
		+

Puja Mody: Autocreate 2012/11/27 Entry for User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles

2012-11-27T19:23:18Z

Autocreate 2012/11/27 Entry for User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles

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