User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/27: Difference between revisions
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* [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>] made on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17| October 17]] was used for the solutions. 8.42mM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] solution and 54μM ADA were used to prepare solutions with 1μM ADA and 25μM, 50μM, 75μM, 100μM, 125μM, 150μM, 175μM, and 200μM HAuCl<sub>4</sub>. Water was used as a solvent and enough was added for each solution to be 2mL. The amount of ADA used was kept constant at 55.19μL. The solutions were capped with tin foil and heated for 4 hours at 80<sup>o</sup>C. | * [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>] made on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17| October 17]] was used for the solutions. 8.42mM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] solution and 54μM ADA were used to prepare solutions with 1μM ADA and 25μM, 50μM, 75μM, 100μM, 125μM, 150μM, 175μM, and 200μM HAuCl<sub>4</sub>. Water was used as a solvent and enough was added for each solution to be 2mL. The amount of ADA used was kept constant at 55.19μL. The solutions were capped with tin foil and heated for 4 hours at 80<sup>o</sup>C. | ||
* Meanwhile we continued to run our ADA Kinetic Assay. We continued to monitor λ 235 but instead of correlating it with adenosine, we monitored the kinetics as the creation of inosine. Please see Mary's notebook for data | * Meanwhile we continued to run our ADA Kinetic Assay. We continued to monitor λ 235 but instead of correlating it with adenosine, we monitored the kinetics as the creation of inosine. Please see Mary's notebook for data. in order to ensure that the absorbance remained below 1, we had to use half as much of the ADA | ||
==Conclusions== | ==Conclusions== |
Revision as of 11:15, 29 November 2012
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Kinetics and DialysisGoals
Procedure
Conclusions
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