User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/27: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* The contents of the dialysis pouches were collected in separate falcon tubes. The two samples of ADA that were run through the dialysis were Frac #2 and Frac #8 from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| 2012/09/26]]. We decided to made the new Au/ADA solutions using ADA frac # 8. We chose it because after New frac, it was the collected fraction that had the highest [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/07| concentration of ADA present]]. | * The contents of the dialysis pouches were collected in separate falcon tubes. The two samples of ADA that were run through the dialysis were Frac #2 and Frac #8 from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| 2012/09/26]]. We decided to made the new Au/ADA solutions using ADA frac # 8. We chose it because after New frac, it was the collected fraction that had the highest [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/07| concentration of ADA present]]. | ||
* [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>] made on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17| October 17]] was used for the solutions. 8.42mM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] solution and 54μM ADA were used to prepare solutions with 1μM ADA and 25μM, 50μM, 75μM, 100μM, 125μM, 150μM, 175μM, and 200μM HAuCl<sub>4</sub>. Water was used as a solvent and enough was added for each solution to be 2mL. The amount of ADA used was kept constant at 55.19μL. The solutions were capped with tin foil and heated for 4 hours at 80<sup>o</sup>C. | * [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] made on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17| October 17]] was used for the solutions. 8.42mM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] solution and 54μM ADA were used to prepare solutions with 1μM ADA and 25μM, 50μM, 75μM, 100μM, 125μM, 150μM, 175μM, and 200μM HAuCl<sub>4</sub>. Water was used as a solvent and enough was added for each solution to be 2mL. The amount of ADA used was kept constant at 55.19μL. The solutions were capped with tin foil and heated for 4 hours at 80<sup>o</sup>C. | ||
* Meanwhile we continued to run our ADA Kinetic Assay. We continued to monitor λ 235 but instead of correlating it with adenosine, we monitored the kinetics as the creation of inosine. | * Meanwhile we continued to run our ADA Kinetic Assay. We continued to monitor λ 235 but instead of correlating it with adenosine, we monitored the kinetics as the creation of inosine. Please see [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/27| Mary's notebook]] for graphs of the spectra. in order to ensure that the absorbance remained below 1, we had to use half as much of the ADA | ||
==Conclusions== | ==Conclusions== | ||
* The Au/ADA solutions made on [[User:Michael F. Nagle/Notebook/Chem 571/2012/11/14| November 14]] were run through the UV-Vis by Mike on Tuesday. Please see his notebook for details on procedure and more observations. The solutions did not produce any nano particle fibers and the no absorbance was detected in the UV-Vis. | * The Au/ADA solutions made on [[User:Michael F. Nagle/Notebook/Chem 571/2012/11/14| November 14]] were run through the UV-Vis by Mike on [[User:Michael F. Nagle/Notebook/Chem 571/2012/11/20| Tuesday]]. Please see his notebook for details on procedure and more observations. The solutions did not produce any nano particle fibers and the no absorbance was detected in the UV-Vis. | ||
Latest revision as of 22:17, 26 September 2017
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Kinetics and DialysisGoals
Procedure
Conclusions
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