User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/14: Difference between revisions
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==Goals== | ==Goals== | ||
* We picked up from yesterday and continued with the [[AU Biomaterials Design Lab:Protocols/ADA Activity Assay| Kinetic Assay protocol]] | * We picked up from yesterday and continued with the [[AU Biomaterials Design Lab:Protocols/ADA Activity Assay| Kinetic Assay protocol]] | ||
* We made solutions of gold in our sample of adenosine deaminase. | * We made solutions of gold in our sample of adenosine deaminase. This procedure and concentrations can be found in [[User:Michael F. Nagle/Notebook/Chem 571/2012/11/14| Michael's notebook]] | ||
* We also began the process of conducting dialysis on our samples of ADA in order to remove the salts from the samples | * We also began the process of conducting dialysis on our samples of ADA in order to remove the salts from the samples | ||
==Procedure== | ==Procedure== | ||
* For all information regarding the | * For all information regarding the procedure followed for the dialysis of the ADA, please see [[User:Michael F. Nagle/Notebook/Chem 571/2012/11/14| Michael's notebook.]] | ||
* From the spectras collected yesterday, we looked at the absorbances of inosine and adenosine at two specific wavelengths, 235nm and 265 nm. These absorbances were used to calculate the molar absorptivity using Beers Law A=εbc | * From the spectras collected yesterday, we looked at the absorbances of inosine and adenosine at two specific wavelengths, 235nm and 265 nm. These absorbances were used to calculate the molar absorptivity using Beers Law A=εbc | ||
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**Inosine: λ235, ε= 7540; λ265 ε= 5530 | **Inosine: λ235, ε= 7540; λ265 ε= 5530 | ||
** This new data shows that what we had been measuring the entire time at the 235nm wavelength was the absorbance of the inosine which was supposed to increase with the addition of the ADA. As a result, we will be continuing with this portion of the experiment in the future. | |||
Revision as of 12:57, 24 November 2012
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Continue ADA kinetics assayGoals
Procedure
(For full calculations, please see Mary's notebook)
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