User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/14: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Procedure== | ==Procedure== | ||
* For all information regarding the | * For all information regarding the procedure followed for the dialysis of the ADA, please see [[User:Michael F. Nagle/Notebook/Chem 571/2012/11/14| Michael's notebook.]] | ||
* From the spectras collected yesterday, we looked at the absorbances of inosine and adenosine at two specific wavelengths, 235nm and 265 nm. These absorbances were used to calculate the molar absorptivity using Beers Law A=εbc | * From the spectras collected yesterday, we looked at the absorbances of inosine and adenosine at two specific wavelengths, 235nm and 265 nm. These absorbances were used to calculate the molar absorptivity using Beers Law A=εbc | ||
** For inosine, at λ235, ε= 1400; λ265 ε= 6000 | ** For inosine, at λ235, ε= 1400; λ265 ε= 6000 | ||
** and for Adenosine at λ235, ε= 18566; λ265 ε= 13029 | ** and for Adenosine at λ235, ε= 18566; λ265 ε= 13029 | ||
(For full calculations, please see Mary's notebook) | (For full calculations, please see [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/14| Mary's notebook]]) | ||
** based on these calculations, which were checked multiple times, the greatest differential was seen to be at λ235, where adenosine has an extremely high molar absorptivity and comparatively, the absorptivity for inosine was relatively low. Based on this fact, in the following samples, we monitored the kinetics of the reaction at a wavelength of 235. in these samples, there was adenosine, ADA and phosphate buffer present. in the catalysis of this reaction, ADA being an enzyme would eat up the adenosine and convert it into inosine. as a result, on our spectra, we expected to see the absorbance of the sample to decrease as time went by. | ** based on these calculations, which were checked multiple times, the greatest differential was seen to be at λ235, where adenosine has an extremely high molar absorptivity and comparatively, the absorptivity for inosine was relatively low. Based on this fact, in the following samples, we monitored the kinetics of the reaction at a wavelength of 235. in these samples, there was adenosine, ADA and phosphate buffer present. in the catalysis of this reaction, ADA being an enzyme would eat up the adenosine and convert it into inosine. as a result, on our spectra, we expected to see the absorbance of the sample to decrease as time went by. |
Latest revision as of 22:15, 26 September 2017
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Procedure
(For full calculations, please see Mary's notebook)
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