User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/13: Difference between revisions
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==Procedure== | ==Procedure== | ||
* For procedure and data regarding the UV-Vis and the AA of the HRP and gold nanoparticle solutions, please see either [[User:Melissa Novy/Notebook/CHEM-571/2012/11/13| Melissa's notebook]] or [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/13| Dhea's notebook.]] | * For procedure and data regarding the UV-Vis and the AA of the HRP and gold nanoparticle solutions, please see either [[User:Melissa Novy/Notebook/CHEM-571/2012/11/13| Melissa's notebook]] or [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/13| Dhea's notebook.]] | ||
* The Adenosine Deaminase Kinetic assay was done by following the Assay [[AU Biomaterials Design Lab:Protocols/ADA Activity Assay| Protocol]] | * The Adenosine Deaminase Kinetic assay was done by following the Assay [[AU Biomaterials Design Lab:Protocols/ADA Activity Assay| Protocol]] | ||
Revision as of 12:55, 24 November 2012
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ADA Kinetics and moreGoals
Procedure
.05 x .05= .0025 moles .0025 moles x 268.07g/1mol = .6702g sodium phosphate in 50 mL H20. Actual amount = .6705. pH of 7.4 was achieve by adding roughly 3 drops of 12M HCl from a glass pasture pipette.
(0.00373 M)(V1)=(5 mL)(0.1×10-3 M) V1 = 0.134 mL
65μM * 3μL= M2 * 1000μL
Data
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