User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/07 - Revision history

Puja Mody: /* DATA */

Puja Mody — Sat, 24 Nov 2012 19:51:46 GMT

DATA

←Older revision		Revision as of 19:51, 24 November 2012
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	\|}		\|}

-	** Frac 2 represents the flasks 2-4 collection done in [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26\| September]]. Frac 8 represents collection of [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26\|flask 1]], and Frac New represents the ADA just made [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06\| ~~last week~~.]]	+	** Frac 2 represents the flasks 2-4 collection done in [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26\| September]]. Frac 8 represents collection of [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26\|flask 1]], and Frac New represents the ADA just made [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06\| yesterday.]]

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Puja Mody: /* DATA */

Puja Mody — Sat, 24 Nov 2012 19:51:15 GMT

DATA

←Older revision		Revision as of 19:51, 24 November 2012
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	\|}		\|}

-	** Frac 2 represents the flasks 2-4 collection done in ~~october~~. Frac 8 represents collection of flask 1, and Frac New represents the ADA just made last week.	+	** Frac 2 represents the flasks 2-4 collection done in [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26\| September]]. Frac 8 represents collection of [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26\|flask 1]], and Frac New represents the ADA just made [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06\| last week.]]

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Puja Mody: /* Procedure */

Puja Mody — Sat, 24 Nov 2012 19:40:16 GMT

Procedure

←Older revision		Revision as of 19:40, 24 November 2012
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-	* For all information regarding the Au/HRP solutions, please see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/07\| Dhea's Notebook]]	+	* For all information regarding the making and analyzing of the Au/HRP solutions , please see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/07\| Dhea's Notebook]]

	==DATA==		==DATA==

Puja Mody: /* DATA */

Puja Mody — Sat, 24 Nov 2012 19:39:38 GMT

DATA

←Older revision		Revision as of 19:39, 24 November 2012
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		+	** Frac 2 represents the flasks 2-4 collection done in october. Frac 8 represents collection of flask 1, and Frac New represents the ADA just made last week.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Puja Mody: /* Goals */

Puja Mody — Sat, 24 Nov 2012 19:38:10 GMT

Goals

←Older revision		Revision as of 19:38, 24 November 2012
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	==Goals==		==Goals==
-	* Conduct a bradford assay on the collected fraction of ADA made on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06\| 2012/11/06]] and on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26\| 2012/09/26]].	+	* Conduct a bradford assay on the collected fraction of ADA made on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06\| 2012/11/06]] and on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26\| 2012/09/26]]. The bradford assay will allow us to calculate the concentration of ADA present in our collected fraction samples.
	*Prepare Au/HRP solutions		*Prepare Au/HRP solutions

Puja Mody: /* Procedure */

Puja Mody — Sat, 24 Nov 2012 19:37:20 GMT

Procedure

←Older revision		Revision as of 19:37, 24 November 2012
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	==Procedure==		==Procedure==
-	* A stock solution of 1mg/mL BSA was prepared ~~by adding 10mg of BSA into a 10mL volumetric flask and then filling it with autoclaved water. The~~ actual concentration ~~of the BSA stock was~~ 1.02mg/mL ~~as the actual weighed out BSA added was~~ 10.2mg BSA.	+	* A stock solution of 1mg/mL BSA was prepared (actual concentration = 1.02mg/mL). 10.2mg BSA added to 10mL of water in a volumetric flask.
	*In order to determine the concentration of the ADA collected in the fracs, standard solutions had to be made and run through the UV-Vis first. Seven standard solutions were prepared with 1X Bradford Reagent, varying amounts of BSA stock, and autoclaved water. These solutions were analyzed in the UV-Vis from 500-800 nm.		*In order to determine the concentration of the ADA collected in the fracs, standard solutions had to be made and run through the UV-Vis first. Seven standard solutions were prepared with 1X Bradford Reagent, varying amounts of BSA stock, and autoclaved water. These solutions were analyzed in the UV-Vis from 500-800 nm.

Puja Mody: /* Procedure */

Puja Mody — Thu, 15 Nov 2012 18:03:20 GMT

Procedure

←Older revision		Revision as of 18:03, 15 November 2012
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	* In a cuvet, 7.5μL of autclaved water, 7.5 μL of a fraction and 715μL 1X bradford Reagent were added. In total 3 cuvet samples were run through the UV-Vis.		* In a cuvet, 7.5μL of autclaved water, 7.5 μL of a fraction and 715μL 1X bradford Reagent were added. In total 3 cuvet samples were run through the UV-Vis.
	**Because the spectra for frac #8 and Frac New had an absorbance of over 1, those cuvet samples were diluted in half using the same procedure as for the standard solutions in order to get an acceptable (below 1) absorbance)		**Because the spectra for frac #8 and Frac New had an absorbance of over 1, those cuvet samples were diluted in half using the same procedure as for the standard solutions in order to get an acceptable (below 1) absorbance)
		+
		+
		+	* For all information regarding the Au/HRP solutions, please see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/07\| Dhea's Notebook]]

	==DATA==		==DATA==

Puja Mody: /* Goals */

Puja Mody — Thu, 15 Nov 2012 18:02:21 GMT

Goals

←Older revision		Revision as of 18:02, 15 November 2012
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	==Goals==		==Goals==
-	* Conduct a bradford assay on the collected fraction of ADA made on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06\| 2012/11/06]] and on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26\| 2012/09/26]].	+	* Conduct a bradford assay on the collected fraction of ADA made on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06\| 2012/11/06]] and on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26\| 2012/09/26]].
		+	*Prepare Au/HRP solutions

	==Procedure==		==Procedure==

Puja Mody: /* DATA */

Puja Mody — Sun, 11 Nov 2012 22:11:48 GMT

DATA

←Older revision		Revision as of 22:11, 11 November 2012
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	\| align="center" style="background:#f0f0f0;"\|'''uM'''		\| align="center" style="background:#f0f0f0;"\|'''uM'''
	\|-		\|-
-	\| F2\|\|0.015\|\|0.93\|\|0.017747193\|\|0.025644694\|\|6.30091E-10\|\|52.40265306\|\|4.20061E-05\|\|42.0060507	+	\| Frac 2\|\|0.015\|\|0.93\|\|0.017747193\|\|0.025644694\|\|6.30091E-10\|\|52.40265306\|\|4.20061E-05\|\|42.0060507
	\|-		\|-
-	\| F8\|\|0.0075\|\|0.757\|\|0.011481347\|\|0.016590547\|\|4.0763E-10\|\|65.93302839\|\|5.43507E-05\|\|54.35068601	+	\| Frac 8\|\|0.0075\|\|0.757\|\|0.011481347\|\|0.016590547\|\|4.0763E-10\|\|65.93302839\|\|5.43507E-05\|\|54.35068601
	\|-		\|-
-	\| ~~Fnew~~\|\|0.0075\|\|0.819\|\|0.013726911\|\|0.019835386\|\|4.87356E-10\|\|59.66382586\|\|6.49808E-05\|\|64.98078863	+	\| Frac new\|\|0.0075\|\|0.819\|\|0.013726911\|\|0.019835386\|\|4.87356E-10\|\|59.66382586\|\|6.49808E-05\|\|64.98078863
	\|}		\|}

Puja Mody: /* DATA */

Puja Mody — Sun, 11 Nov 2012 22:11:16 GMT

DATA

←Older revision		Revision as of 22:11, 11 November 2012
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	Concentrations, absorbances, for the collected fracs of ADA		Concentrations, absorbances, for the collected fracs of ADA

-	Sample Name Volume of ADA[mL] Absorbance [ADA] [mg/mL] Mass of ADA[mg] Mols of ADA [mol] Molar absorptivity [mL/mg*cm-1] molarity[M] uM	+	{\| {{table}}
-	F2 0.015 0.93 0.017747193 0.025644694 6.30091E-10 52.40265306 4.20061E-05 42.0060507	+	\| align="center" style="background:#f0f0f0;"\|'''Sample Name'''
-	F8 0.0075 0.757 0.011481347 0.016590547 4.0763E-10 65.93302839 5.43507E-05 54.35068601	+	\| align="center" style="background:#f0f0f0;"\|'''Volume of ADA[mL]'''
-	Fnew 0.0075 0.819 0.013726911 0.019835386 4.87356E-10 59.66382586 6.49808E-05 64.98078863	+	\| align="center" style="background:#f0f0f0;"\|'''Absorbance'''
		+	\| align="center" style="background:#f0f0f0;"\|'''[ADA] [mg/mL]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Mass of ADA[mg]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Mols of ADA [mol]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Molar absorptivity [mL/mg*cm-1]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''molarity[M]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''uM'''
		+	\|-
		+	\| F2\|\|0.015\|\|0.93\|\|0.017747193\|\|0.025644694\|\|6.30091E-10\|\|52.40265306\|\|4.20061E-05\|\|42.0060507
		+	\|-
		+	\| F8\|\|0.0075\|\|0.757\|\|0.011481347\|\|0.016590547\|\|4.0763E-10\|\|65.93302839\|\|5.43507E-05\|\|54.35068601
		+	\|-
		+	\| Fnew\|\|0.0075\|\|0.819\|\|0.013726911\|\|0.019835386\|\|4.87356E-10\|\|59.66382586\|\|6.49808E-05\|\|64.98078863
		+	\|}