User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/07: Difference between revisions
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==Goals== | ==Goals== | ||
* Conduct a bradford assay on the collected fraction of ADA made on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06| 2012/11/06]] and on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| 2012/09/26]]. | * Conduct a bradford assay on the collected fraction of ADA made on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06| 2012/11/06]] and on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| 2012/09/26]]. The bradford assay will allow us to calculate the concentration of ADA present in our collected fraction samples. | ||
*Prepare Au/HRP solutions | *Prepare Au/HRP solutions | ||
==Procedure== | ==Procedure== | ||
* A stock solution of 1mg/mL BSA was prepared | * A stock solution of 1mg/mL BSA was prepared (actual concentration = 1.02mg/mL). 10.2mg BSA added to 10mL of water in a volumetric flask. | ||
*In order to determine the concentration of the ADA collected in the fracs, standard solutions had to be made and run through the UV-Vis first. Seven standard solutions were prepared with 1X Bradford Reagent, varying amounts of BSA stock, and autoclaved water. These solutions were analyzed in the UV-Vis from 500-800 nm. | *In order to determine the concentration of the ADA collected in the fracs, standard solutions had to be made and run through the UV-Vis first. Seven standard solutions were prepared with 1X Bradford Reagent, varying amounts of BSA stock, and autoclaved water. These solutions were analyzed in the UV-Vis from 500-800 nm. | ||
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* For all information regarding the Au/HRP solutions, please see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/07| Dhea's Notebook]] | * For all information regarding the making and analyzing of the Au/HRP solutions , please see [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/07| Dhea's Notebook]] | ||
==DATA== | ==DATA== | ||
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** Frac 2 represents the flasks 2-4 collection done in [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| September]]. Frac 8 represents collection of [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26|flask 1]], and Frac New represents the ADA just made [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06| yesterday.]] | |||
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Revision as of 12:51, 24 November 2012
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Bradford AssayGoals
Procedure
DATA
Concentrations, absorbances, for the collected fracs of ADA
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