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Project name
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FPLC and Transformation
Goals
- Run Fast Protein Liquid Chromatography (FPLC)
- Redo cell transformation
Procedure
- Please refer to the protocol that was previously employed. No changes were made in this procedure. 3 fracs of ADA were collected. Frac 2 had the most protein present with small amounts present in fracs 1 and 3.
- The transformation and plating of PCR product that was done on 2012/10/24 was unsuccessful and had to be repeated. the Original protocol for PCR mutation can be found here. The following modifications were made:
- 20μL DNA solution was added to 30μL solution with E. Coli cells.
- 250μL Lysogeny Broth (LB) was used instead of SOC medium.
- Single 200μL plates were prepared. No 50μL plates were made.
Conclusions
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