User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06 - Revision history

Puja Mody: /* Procedure */

Puja Mody — Sat, 24 Nov 2012 19:34:38 GMT

Procedure

←Older revision		Revision as of 19:34, 24 November 2012
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	==Procedure==		==Procedure==
-	* Please refer to the [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26\| ~~protocol~~]] that was previously employed. No changes were made in this procedure. 3 fracs of ADA were collected. Frac 2 had the most protein present with small amounts present in fracs 1 and 3.	+	* Please refer to the FPLC [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26\| instructions]] that was previously employed. No changes were made in this procedure. 3 fracs of ADA were collected. Frac 2 had the most protein present with small amounts present in fracs 1 and 3. The only difference this time was that because there were not two separate samples of protein, everything could be run through the FPLC at once. the flow rate was 5mL/minute
	* The transformation and plating of PCR product that was done on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/24\| 2012/10/24]] was unsuccessful and had to be repeated. the Original protocol for PCR mutation can be found [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol\| here]]. The following modifications were made:		* The transformation and plating of PCR product that was done on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/24\| 2012/10/24]] was unsuccessful and had to be repeated. the Original protocol for PCR mutation can be found [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol\| here]]. The following modifications were made:
	**20μL DNA solution was added to 30μL solution with E. Coli cells.		**20μL DNA solution was added to 30μL solution with E. Coli cells.

Puja Mody at 21:19, 11 November 2012

Puja Mody — Sun, 11 Nov 2012 21:19:59 GMT

←Older revision		Revision as of 21:19, 11 November 2012
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	==Goals==		==Goals==
	*Run Fast Protein Liquid Chromatography (FPLC)		*Run Fast Protein Liquid Chromatography (FPLC)
-	*~~Transform Adenosine Deaminase~~ in cells	+	*Redo cell transformation
		+
		+	==Procedure==
		+	* Please refer to the [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26\| protocol]] that was previously employed. No changes were made in this procedure. 3 fracs of ADA were collected. Frac 2 had the most protein present with small amounts present in fracs 1 and 3.
		+	* The transformation and plating of PCR product that was done on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/24\| 2012/10/24]] was unsuccessful and had to be repeated. the Original protocol for PCR mutation can be found [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol\| here]]. The following modifications were made:
		+	**20μL DNA solution was added to 30μL solution with E. Coli cells.
		+	**250μL Lysogeny Broth (LB) was used instead of SOC medium.
		+	**Single 200μL plates were prepared. No 50μL plates were made.
		+
		+	==Conclusions==
		+

Puja Mody at 21:12, 11 November 2012

Puja Mody — Sun, 11 Nov 2012 21:12:07 GMT

←Older revision		Revision as of 21:12, 11 November 2012
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	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==FPLC and Transformation==
-	* ~~Insert content here...~~	+
		+	==Goals==
		+	*Run Fast Protein Liquid Chromatography (FPLC)
		+	*Transform Adenosine Deaminase in cells

Puja Mody: Autocreate 2012/11/06 Entry for User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles

Puja Mody — Sun, 11 Nov 2012 21:05:53 GMT

Autocreate 2012/11/06 Entry for User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles

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