User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

FPLC and Transformation

Goals

  • Run Fast Protein Liquid Chromatography (FPLC)
  • Redo cell transformation

Procedure

  • Please refer to the protocol that was previously employed. No changes were made in this procedure. 3 fracs of ADA were collected. Frac 2 had the most protein present with small amounts present in fracs 1 and 3.
  • The transformation and plating of PCR product that was done on 2012/10/24 was unsuccessful and had to be repeated. the Original protocol for PCR mutation can be found here. The following modifications were made:
    • 20μL DNA solution was added to 30μL solution with E. Coli cells.
    • 250μL Lysogeny Broth (LB) was used instead of SOC medium.
    • Single 200μL plates were prepared. No 50μL plates were made.

Conclusions


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/11/06&oldid=653224"