User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06

(Difference between revisions)

Revision as of 17:19, 11 November 2012

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Please refer to the protocol that was previously employed. No changes were made in this procedure. 3 fracs of ADA were collected. Frac 2 had the most protein present with small amounts present in fracs 1 and 3.
The transformation and plating of PCR product that was done on 2012/10/24 was unsuccessful and had to be repeated. the Original protocol for PCR mutation can be found here. The following modifications were made:
- 20μL DNA solution was added to 30μL solution with E. Coli cells.
- 250μL Lysogeny Broth (LB) was used instead of SOC medium.
- Single 200μL plates were prepared. No 50μL plates were made.

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 ==Goals==
 *Run Fast Protein Liquid Chromatography (FPLC)
-*Transform Adenosine Deaminase in cells
+*Redo cell transformation
+==Procedure==
+* Please refer to the [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| protocol]] that was previously employed. No changes were made in this procedure. 3 fracs of ADA were collected. Frac 2 had the most protein present with small amounts present in fracs 1 and 3.
+* The transformation and plating of PCR product that was done on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/24| 2012/10/24]] was unsuccessful and had to be repeated. the Original protocol for PCR mutation can be found [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol| here]]. The following modifications were made:
+**20μL DNA solution was added to 30μL solution with E. Coli cells.
+**250μL Lysogeny Broth (LB) was used instead of SOC medium.
+**Single 200μL plates were prepared. No 50μL plates were made.
+==Conclusions==