User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06

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==Goals==
==Goals==
*Run Fast Protein Liquid Chromatography (FPLC)
*Run Fast Protein Liquid Chromatography (FPLC)
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*Transform Adenosine Deaminase in cells
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*Redo cell transformation
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==Procedure==
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* Please refer to the [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| protocol]] that was previously employed. No changes were made in this procedure. 3 fracs of ADA were collected. Frac 2 had the most protein present with small amounts present in fracs 1 and 3.
 +
* The transformation and plating of PCR product that was done on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/24| 2012/10/24]] was unsuccessful and had to be repeated. the Original protocol for PCR mutation can be found [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol| here]]. The following modifications were made:
 +
**20μL DNA solution was added to 30μL solution with E. Coli cells.
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**250μL Lysogeny Broth (LB) was used instead of SOC medium.
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**Single 200μL plates were prepared. No 50μL plates were made.
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==Conclusions==
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Revision as of 16:19, 11 November 2012

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FPLC and Transformation

Goals

  • Run Fast Protein Liquid Chromatography (FPLC)
  • Redo cell transformation

Procedure

  • Please refer to the protocol that was previously employed. No changes were made in this procedure. 3 fracs of ADA were collected. Frac 2 had the most protein present with small amounts present in fracs 1 and 3.
  • The transformation and plating of PCR product that was done on 2012/10/24 was unsuccessful and had to be repeated. the Original protocol for PCR mutation can be found here. The following modifications were made:
    • 20μL DNA solution was added to 30μL solution with E. Coli cells.
    • 250μL Lysogeny Broth (LB) was used instead of SOC medium.
    • Single 200μL plates were prepared. No 50μL plates were made.

Conclusions


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