User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/31 - Revision history

Puja Mody: /* Procedure */

Puja Mody — Sat, 24 Nov 2012 19:32:39 GMT

Procedure

←Older revision		Revision as of 19:32, 24 November 2012
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	==Procedure==		==Procedure==
-	*The same procedure from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/25\| 2012/09/25]] was followed in order to separate out the proteins from the cell organelles.	+	*The same procedure from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/25\| 2012/09/25]] was followed in order to separate out the proteins from the cell organelles.
-	* For information regarding the AuNP lysozyme UV-Vis spectras please see [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/31\| Michael's Notebook]].	+	** The cells were shocked open by alternating sonication for 30 seconds followed by 30 seconds on ice.
		+	**Afterwards, the cells were centrifuged at 18000 rpm and 4°C for 2 hours.
		+	**The supernatant was collected and purified through vacuum filtration with filter pape membrane in which the holes measure 450nm. The filtered product was collected and stored in the refrigerator for approximately 1 week.
		+
		+	* For information regarding the AuNP lysozyme UV-Vis spectras please see [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/31\| Michael's Notebook]]. These spectras were taken using the same samples as were made [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/24\| last week]]

	==Data==		==Data==

Puja Mody at 17:59, 15 November 2012

Puja Mody — Thu, 15 Nov 2012 17:59:42 GMT

←Older revision		Revision as of 17:59, 15 November 2012
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	*The same procedure from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/25\| 2012/09/25]] was followed in order to separate out the proteins from the cell organelles.		*The same procedure from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/25\| 2012/09/25]] was followed in order to separate out the proteins from the cell organelles.
	* For information regarding the AuNP lysozyme UV-Vis spectras please see [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/31\| Michael's Notebook]].		* For information regarding the AuNP lysozyme UV-Vis spectras please see [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/31\| Michael's Notebook]].
		+
		+	==Data==
		+
		+	[[Image:UV-Vis AuNP in lysozyme.png\| UV-Vis runs of AuNP in Lysozyme]]
		+
		+	[[Image:Redone quartz cuvets.png\| Redone samples in Quartz Cuvets]]

	==Conclusions==		==Conclusions==
	* The separation process ran smoothly except for during the vacuum filtration. There seemed to be a defect in the clamp being used which caused some of the supernatant collected to leak out. A glass pipette was used to recollect as much as possible and placed back through the filter. This defect may be the cause for any loss in ADA collected when conducting the FPLC.		* The separation process ran smoothly except for during the vacuum filtration. There seemed to be a defect in the clamp being used which caused some of the supernatant collected to leak out. A glass pipette was used to recollect as much as possible and placed back through the filter. This defect may be the cause for any loss in ADA collected when conducting the FPLC.
		+	* Samples 134-140 showed a variation in the baseline and as a result, in order to determine whether this was due to lysozyme or a variation int he cuvettes, these four trials were repeated using the same quartz cuvet. A blank of water was run using the same cuvette and subtracted from each spectra manually.

Puja Mody at 21:05, 11 November 2012

Puja Mody — Sun, 11 Nov 2012 21:05:17 GMT

←Older revision		Revision as of 21:05, 11 November 2012
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	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Separate Protein and Lysozyme UV-Vis==
-	* ~~Insert content here~~...	+
		+	==Goals==
		+	*Separate the proteins from the cells
		+	* Run UV-Vis on the AuNP/Lysozyme solutions
		+
		+	==Procedure==
		+	*The same procedure from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/25\| 2012/09/25]] was followed in order to separate out the proteins from the cell organelles.
		+	* For information regarding the AuNP lysozyme UV-Vis spectras please see [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/31\| Michael's Notebook]].
		+
		+	==Conclusions==
		+	* The separation process ran smoothly except for during the vacuum filtration. There seemed to be a defect in the clamp being used which caused some of the supernatant collected to leak out. A glass pipette was used to recollect as much as possible and placed back through the filter. This defect may be the cause for any loss in ADA collected when conducting the FPLC.

Puja Mody: Autocreate 2012/10/31 Entry for User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles

Puja Mody — Sun, 11 Nov 2012 18:08:47 GMT

Autocreate 2012/10/31 Entry for User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles

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